Literature DB >> 22635996

Reclamation of ampicillin sensitivity for the genetic manipulation of Legionella pneumophila.

Molly C Sutherland1, Joseph P Vogel.   

Abstract

Research on Legionella pneumophila, the causative agent of Legionnaires' disease, has been hampered due to the lack of selectable markers for genetic manipulation. We report the construction of a mutant strain of L. pneumophila lacking loxA, a chromosomally encoded β-lactamase, that has enhanced sensitivity to ampicillin. Also described are a method for converting Legionella strains to ampicillin sensitivity and conditions for utilizing bla as a selectable marker.

Entities:  

Mesh：

Substances：

Year:  2012        PMID： 22635996      PMCID： PMC3416426          DOI： 10.1128/AEM.00669-12

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  22 in total

1.  The Legionella (Fluoribacter) gormanii metallo-beta-lactamase: a new member of the highly divergent lineage of molecular-subclass B3 beta-lactamases.

Authors:  L Boschi; P S Mercuri; M L Riccio; G Amicosante; M Galleni; J M Frère; G M Rossolini
Journal:  Antimicrob Agents Chemother       Date:  2000-06       Impact factor: 5.191

2.  Regulation of hypercompetence in Legionella pneumophila.

Authors:  Jessica A Sexton; Joseph P Vogel
Journal:  J Bacteriol       Date:  2004-06       Impact factor: 3.490

3.  Inactivation of beta-lactam antibiotics by Legionella pneumophila.

Authors:  K P Fu; H C Neu
Journal:  Antimicrob Agents Chemother       Date:  1979-11       Impact factor: 5.191

Review 4.  Legionella spp. and Legionnaires' disease.

Authors:  B M W Diederen
Journal:  J Infect       Date:  2007-11-05       Impact factor: 6.072

5.  Sequence and genome context analysis of a new molecular class D beta-lactamase gene from Legionella pneumophila.

Authors:  Matthew B Avison; Alan M Simm
Journal:  J Antimicrob Chemother       Date:  2002-09       Impact factor: 5.790

6.  Characterization of the beta-lactamases of six species of Legionella.

Authors:  R Marre; A A Medeiros; A W Pasculle
Journal:  J Bacteriol       Date:  1982-07       Impact factor: 3.490

Review 7.  Legionella and Legionnaires' disease: 25 years of investigation.

Authors:  Barry S Fields; Robert F Benson; Richard E Besser
Journal:  Clin Microbiol Rev       Date:  2002-07       Impact factor: 26.132

8.  Legionnaires' disease: isolation of a bacterium and demonstration of its role in other respiratory disease.

Authors:  J E McDade; C C Shepard; D W Fraser; T R Tsai; M A Redus; W R Dowdle
Journal:  N Engl J Med       Date:  1977-12-01       Impact factor: 91.245

9.  Biochemical properties of beta-lactamase produced by Legionella gormanii.

Authors:  T Fujii; K Sato; K Miyata; M Inoue; S Mitsuhashi
Journal:  Antimicrob Agents Chemother       Date:  1986-05       Impact factor: 5.191

10.  Susceptibility of Legionella pneumophila to twenty antimicrobial agents.

Authors:  P H Edelstein; R D Meyer
Journal:  Antimicrob Agents Chemother       Date:  1980-09       Impact factor: 5.191
View more
  1 in total

1.  Reassessing the role of DotF in the Legionella pneumophila type IV secretion system.

Authors:  Molly C Sutherland; Kelsey A Binder; Phillip Y Cualing; Joseph P Vogel
Journal:  PLoS One       Date:  2013-06-07       Impact factor: 3.240
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.