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Literature DB >> 2263451

Interaction of the isolated domain II/III of Thermus thermophilus elongation factor Tu with the nucleotide exchange factor EF-Ts.

M E Peter¹, C O Reiser, N K Schirmer, T Kiefhaber, G Ott, N W Grillenbeck, M Sprinzl.

Abstract

The middle and C-terminal domain (domain II/III) of elongation factor Tu from Thermus thermophilus lacking the GTP/GDP binding domain have been prepared by treating nucleotide-free protein with Staphylococcus aureus V8 protease. The isolated domain II/III of EF-Tu has a compact structure and high resistance against tryptic treatment and thermal denaturation. As demonstrated by circular dichroism spectroscopy, the isolated domain II/III does not contain any alpha-helical structure. Nucleotide exchange factor, EF-Ts, was found to interact with domain II/III, whereas the binding of aminoacyl-tRNA, GDP and GTP to this EF-Tu fragment could not be detected.

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Year: 1990 PMID： 2263451 PMCID： PMC332746 DOI： 10.1093/nar/18.23.6889

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

34 in total

1. Structural determination of the functional sites of E. coli elongation factor Tu.

Authors: B F Clark; M Kjeldgaard; T F la Cour; S Thirup; J Nyborg
Journal: Biochim Biophys Acta Date: 1990-08-27

2. Conformational alteration of protein synthesis elongation factor EF-Tu by EF-Ts and by kirromycin.

Authors: T Blumenthal; J Douglass; D Smith
Journal: Proc Natl Acad Sci U S A Date: 1977-08 Impact factor: 11.205

3. Enzymic binding of aminoacyl-tRNA to Escherichia coli ribosomes using modified tRNA species and tRNA fragments.

Authors: T Wagner; M Sprinzl
Journal: Methods Enzymol Date: 1979 Impact factor: 1.600

4. Spectrophotometric determination of protein concentration in cell extracts containing tRNA's and rRNA's.

Authors: B Ehresmann; P Imbault; J H Weil
Journal: Anal Biochem Date: 1973-08 Impact factor: 3.365

5. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

6. Determination of protein secondary structure in solution by vacuum ultraviolet circular dichroism.

Authors: S Brahms; J Brahms
Journal: J Mol Biol Date: 1980-04 Impact factor: 5.469

7. A computer-assisted model for estimating protein secondary structure from circular dichroic spectra: comparison of animal lactate dehydrogenases.

Authors: J B Siegel; W E Steinmetz; G L Long
Journal: Anal Biochem Date: 1980-05-01 Impact factor: 3.365

8. Studies on polypeptide-chain-elongation factors from an extreme thermophile, Thermus thermophilus HB8. 3. Molecular properties.

Authors: S Nakamura; S Ohta; K Arai; N Arai; T Oshima; Y Kaziro
Journal: Eur J Biochem Date: 1978-12

9. Studies on polypeptide-chain-elongation factors from an extreme thermophile, Thermus thermophilus HB8. 1. Purification and some properties of the purified factors.

Authors: K Arai; Y Ota; N Arai; S Nakamura; C Henneke; T Oshima; Y Kaziro
Journal: Eur J Biochem Date: 1978-12

10. Aminoacyl transfer ribonucleic acid binding site of the bacterial elongation factor Tu.

Authors: A Pingoud; C Urbanke
Journal: Biochemistry Date: 1980-05-13 Impact factor: 3.162

4 in total

1. A single amino acid substitution in elongation factor Tu disrupts interaction between the ternary complex and the ribosome.

Authors: I Tubulekas; D Hughes
Journal: J Bacteriol Date: 1993-01 Impact factor: 3.490