PURPOSE: The present study investigates the inhibition of Ultraviolet B (UVB, 290-320 nm) radiation-induced oxidative damage in peripheral blood human lymphocytes by embelin extracted from Embelia ribes. MATERIALS AND METHODS: Embelin was extracted, purified and characterized. Prior to inhibitory assessment, a maximum concentration of embelin that was non-toxic was determined. Six experimental groups, including respective controls were made to assess the inhibitory effect of embelin for the selected concentrations of 10 and 20 μg/ml. For the experimental groups; lymphocytes (1 × 10(6) cells) were pre-treated with the chosen concentration of embelin for a period of 60 min and then exposed to UVB for 30 min. UVB radiation inhibitory effect of embelin assessed by measuring antioxidant and lipid peroxidation levels, deoxyribonucleic acid (DNA) damage, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) at scheduled time points after irradiation. RESULTS: Pre-treatment of lymphocytes with embelin prevents UVB-induced oxidative damage. An increase in antioxidant levels in irradiated cells in the presence of embelin and UV absorbance of embelin could be the reason for the decrease in lipid peroxidation level and prevention of DNA damage by UVB radiation. CONCLUSION: Embelin prevents oxidative stress induced by UVB irradiation via its antioxidant property.
PURPOSE: The present study investigates the inhibition of Ultraviolet B (UVB, 290-320 nm) radiation-induced oxidative damage in peripheral blood human lymphocytes by embelin extracted from Embelia ribes. MATERIALS AND METHODS:Embelin was extracted, purified and characterized. Prior to inhibitory assessment, a maximum concentration of embelin that was non-toxic was determined. Six experimental groups, including respective controls were made to assess the inhibitory effect of embelin for the selected concentrations of 10 and 20 μg/ml. For the experimental groups; lymphocytes (1 × 10(6) cells) were pre-treated with the chosen concentration of embelin for a period of 60 min and then exposed to UVB for 30 min. UVB radiation inhibitory effect of embelin assessed by measuring antioxidant and lipid peroxidation levels, deoxyribonucleic acid (DNA) damage, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) at scheduled time points after irradiation. RESULTS: Pre-treatment of lymphocytes with embelin prevents UVB-induced oxidative damage. An increase in antioxidant levels in irradiated cells in the presence of embelin and UV absorbance of embelin could be the reason for the decrease in lipid peroxidation level and prevention of DNA damage by UVB radiation. CONCLUSION:Embelin prevents oxidative stress induced by UVB irradiation via its antioxidant property.
Authors: H M Kumaraswamy; V Krishna; R Sharath; N D Satyanarayan; P Meghana; R Sandeep Kumar Jain; N Prashanth; H Raja Naika Journal: 3 Biotech Date: 2021-12-08 Impact factor: 2.406