Literature DB >> 22624884

A high-throughput method for screening Arabidopsis mutants with disordered abiotic stress-induced calcium signal.

Zhen Pan1, Yang Zhao, Yuan Zheng, Juntao Liu, Xiangning Jiang, Yan Guo.   

Abstract

It is established that different stresses cause signal-specific changes in cellular Ca(2+) level, which function as messengers in modulating diverse physiological processes. These calcium signals are important for stress adaptation. Though numbers of downstream components of calcium signal cascades have been identified, upstream events in calcium signal remain elusive, specifically components required for calcium signal generation due to the lack of high-throughput genetic assay. Here, we report the development of an easy and efficient method in a forward genetic screen for Ca(2+) signals-deficient mutants in Arabidopsis thaliana. Using this method, 121 mutants with disordered NaCl- and H(2)O(2)-induced Ca(2+) signals are isolated.
Copyright © 2012. Published by Elsevier Ltd.

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Year:  2012        PMID: 22624884     DOI: 10.1016/j.jgg.2012.04.002

Source DB:  PubMed          Journal:  J Genet Genomics        ISSN: 1673-8527            Impact factor:   4.275


  9 in total

Review 1.  Plant calcium-permeable channels.

Authors:  Stéphanie M Swarbreck; Renato Colaço; Julia M Davies
Journal:  Plant Physiol       Date:  2013-07-16       Impact factor: 8.340

2.  The actin-related Protein2/3 complex regulates mitochondrial-associated calcium signaling during salt stress in Arabidopsis.

Authors:  Yi Zhao; Zhen Pan; Yan Zhang; Xiaolu Qu; Yuguo Zhang; Yongqing Yang; Xiangning Jiang; Shanjin Huang; Ming Yuan; Karen S Schumaker; Yan Guo
Journal:  Plant Cell       Date:  2013-11-26       Impact factor: 11.277

3.  Aequorin-based luminescence imaging reveals stimulus- and tissue-specific Ca2+ dynamics in Arabidopsis plants.

Authors:  Xiaohong Zhu; Ying Feng; Gaimei Liang; Na Liu; Jian-Kang Zhu
Journal:  Mol Plant       Date:  2013-01-31       Impact factor: 13.164

Review 4.  Enlightenment on the aequorin-based platform for screening Arabidopsis stress sensory channels related to calcium signaling.

Authors:  Zhiming Yu; Jemma L Taylor; Yue He; Jun Ni
Journal:  Plant Signal Behav       Date:  2015

5.  Measuring spatial and temporal Ca2+ signals in Arabidopsis plants.

Authors:  Xiaohong Zhu; Aaron Taylor; Shenyu Zhang; Dayong Zhang; Ying Feng; Gaimei Liang; Jian-Kang Zhu
Journal:  J Vis Exp       Date:  2014-09-02       Impact factor: 1.355

6.  Abiotic Stress Phenotypes Are Associated with Conserved Genes Derived from Transposable Elements.

Authors:  Zoé Joly-Lopez; Ewa Forczek; Emilio Vello; Douglas R Hoen; Akiko Tomita; Thomas E Bureau
Journal:  Front Plant Sci       Date:  2017-11-28       Impact factor: 5.753

7.  Transcriptome and Metabolome Analyses Revealed the Response Mechanism of Sugar Beet to Salt Stress of Different Durations.

Authors:  Jie Cui; Junliang Li; Cuihong Dai; Liping Li
Journal:  Int J Mol Sci       Date:  2022-08-24       Impact factor: 6.208

8.  Overexpression of Cassava MeAnn2 Enhances the Salt and IAA Tolerance of Transgenic Arabidopsis.

Authors:  Xuejun Lin; Ruimei Li; Yangjiao Zhou; Fenlian Tang; Yajie Wang; Xiaohua Lu; Shijia Wang; Yuan Yao; Jiao Liu; Xinwen Hu; Jianchun Guo
Journal:  Plants (Basel)       Date:  2021-05-08

9.  Screening for Arabidopsis mutants with altered Ca2+ signal response using aequorin-based Ca2+ reporter system.

Authors:  Shujing Sun; Xiaoyan Zhang; Kong Chen; Xiaohong Zhu; Yang Zhao
Journal:  STAR Protoc       Date:  2021-05-23
  9 in total

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