PREMISE OF THE STUDY: Primers were designed for amplifying intron 1 of the single-copy nuclear LEAFY gene for species of Davalliaceae. METHODS AND RESULTS: New primer sets were designed and successfully amplified for intron 1 of the LEAFY gene in 13 species representing the five genera of Davalliaceae. The orthology of these sequences was further confirmed by phylogenetic analyses. Site variation in LEAFY intron 1 sequences across genera of the Davalliaceae and among accessions of the Humata repens complex were 18% and 8%, respectively. Such variation was greater than that for the cpDNA atpB-rbcL intergenic spacer region across the same taxa and accessions. CONCLUSIONS: Using our newly designed primers, intron 1 of the LEAFY gene could be amplified for all species tested. In addition, this single-copy, biparentally inherited, and quickly evolving region showed considerable potential for addressing infraspecific-level questions.
PREMISE OF THE STUDY: Primers were designed for amplifying intron 1 of the single-copy nuclear LEAFY gene for species of Davalliaceae. METHODS AND RESULTS: New primer sets were designed and successfully amplified for intron 1 of the LEAFY gene in 13 species representing the five genera of Davalliaceae. The orthology of these sequences was further confirmed by phylogenetic analyses. Site variation in LEAFY intron 1 sequences across genera of the Davalliaceae and among accessions of the Humata repens complex were 18% and 8%, respectively. Such variation was greater than that for the cpDNA atpB-rbcL intergenic spacer region across the same taxa and accessions. CONCLUSIONS: Using our newly designed primers, intron 1 of the LEAFY gene could be amplified for all species tested. In addition, this single-copy, biparentally inherited, and quickly evolving region showed considerable potential for addressing infraspecific-level questions.
Authors: Carl J Rothfels; Anders Larsson; Fay-Wei Li; Erin M Sigel; Layne Huiet; Dylan O Burge; Markus Ruhsam; Sean W Graham; Dennis W Stevenson; Gane Ka-Shu Wong; Petra Korall; Kathleen M Pryer Journal: PLoS One Date: 2013-10-08 Impact factor: 3.240
Authors: Paul G Wolf; Tanner A Robison; Matthew G Johnson; Michael A Sundue; Weston L Testo; Carl J Rothfels Journal: Appl Plant Sci Date: 2018-05-17 Impact factor: 1.936