Bin Yang1, Xiaqin Sun, Hilal Lashuel, Yan Zhang. 1. State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
Abstract
OBJECTIVE: To investigate whether intracellular amyloid β (iAβ) induces toxicity in wild type (WT) and APP/PS1 mice, a mouse model of Alzheimer's disease. METHODS: Different forms of Aβ aggregates were microinjected into cultured WT or APP/PS1 mouse hippocampal neurons. TUNEL staining was performed to examine neuronal cell death. Reactive oxidative species (ROS) were measured by MitoSOXRed mitochondrial superoxide indicator. RESULTS: Crude, monomer and protofibril Aβ induced more toxicity in APP/PS1 neurons than in WT neurons. ROS are involved in mediating the vulnerability of APP/PS1 neurons to iAβ toxicity. CONCLUSION: Oxidative stress may mediate cell death induced by iAβ in neurons.
OBJECTIVE: To investigate whether intracellular amyloid β (iAβ) induces toxicity in wild type (WT) and APP/PS1mice, a mouse model of Alzheimer's disease. METHODS: Different forms of Aβ aggregates were microinjected into cultured WT or APP/PS1mouse hippocampal neurons. TUNEL staining was performed to examine neuronal cell death. Reactive oxidative species (ROS) were measured by MitoSOXRed mitochondrial superoxide indicator. RESULTS: Crude, monomer and protofibril Aβ induced more toxicity in APP/PS1 neurons than in WT neurons. ROS are involved in mediating the vulnerability of APP/PS1 neurons to iAβ toxicity. CONCLUSION: Oxidative stress may mediate cell death induced by iAβ in neurons.
Authors: G K Gouras; J Tsai; J Naslund; B Vincent; M Edgar; F Checler; J P Greenfield; V Haroutunian; J D Buxbaum; H Xu; P Greengard; N R Relkin Journal: Am J Pathol Date: 2000-01 Impact factor: 4.307
Authors: O Wirths; G Multhaup; C Czech; V Blanchard; S Moussaoui; G Tremp; L Pradier; K Beyreuther; T A Bayer Journal: Neurosci Lett Date: 2001-06-22 Impact factor: 3.046
Authors: Gerd Multhaup; Stefan Scheuermann; Andrea Schlicksupp; Andreas Simons; Markus Strauss; André Kemmling; Christian Oehler; Roberto Cappai; Rüdiger Pipkorn; Thomas A Bayer Journal: Free Radic Biol Med Date: 2002-07-01 Impact factor: 7.376
Authors: Panagiotis Zis; Patrick McHugh; Andrew McQuillin; Domenico Praticò; Mark Dickinson; Sima Shende; Zuzana Walker; Andre Strydom Journal: PLoS One Date: 2014-06-05 Impact factor: 3.240