Literature DB >> 2261918

Antigenicity of fractions of Helicobacter pylori prepared by fast protein liquid chromatography and urease captured by monoclonal antibodies.

A R Stacey1, P R Hawtin, D G Newell.   

Abstract

The antigenicity of Helicobacter pylori protein fractions separated by fast protein liquid chromatography size exclusion was investigated by EIA with sera from patients of well defined Helicobacter pylori status. The antigenic material of Helicobacter pylori was confined to fractions 8 and 14 to 21. Urease containing fractions (14/15) and flagella containing fractions (17/18) were identified. Fraction 8 non-specifically bound human immunoglobulin as demonstrated by the binding of Helicobacter pylori negative sera. The remaining fractions 14 to 21 when used individually as EIA antigens were 91-100% specific, however fractions 16 to 19 showed a reduced sensitivity (78%) compared with the acid extract (95%). The urease fractions were 91% sensitive. Purified urease antigen captured by antiurease monoclonal antibodies was 83% sensitive and 93.3% specific.

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Year:  1990        PMID： 2261918     DOI： 10.1007/bf02184685

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  15 in total

1.  Monoclonal antibodies directed against the flagella of Campylobacter jejuni: production, characterization and lack of effect on the colonization of infant mice.

Authors:  D G Newell
Journal:  J Hyg (Lond)       Date:  1986-04

2.  Antigens for the serodiagnosis of Campylobacter pylori infections.

Authors:  D G Newell; A Stacey
Journal:  Gastroenterol Clin Biol       Date:  1989

3.  Comparison of different antigen preparations in an evaluation of the immune response to Campylobacter pylori.

Authors:  A M Hirschl; M Pletschette; M H Hirschl; J Berger; G Stanek; M L Rotter
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1988-08       Impact factor: 3.267

4.  Herpes simplex-specific IgG subclass response in herpetic keratitis.

Authors:  B W McBride; K A Ward
Journal:  J Med Virol       Date:  1987-02       Impact factor: 2.327

5.  Comparison of ELISA antigen preparations alone or in combination for serodiagnosing Helicobacter pylori infections.

Authors:  A M Hirschl; B J Rathbone; J I Wyatt; J Berger; M L Rotter
Journal:  J Clin Pathol       Date:  1990-06       Impact factor: 3.411

6.  Identification of the outer membrane proteins of Campylobacter pyloridis and antigenic cross-reactivity between C. pyloridis and C. jejuni.

Authors:  D G Newell
Journal:  J Gen Microbiol       Date:  1987-01

7.  An enzyme-linked immunosorbent assay for the serodiagnosis of Campylobacter pylori-associated gastritis.

Authors:  D G Newell; B J Johnston; M H Ali; P I Reed
Journal:  Scand J Gastroenterol Suppl       Date:  1988

8.  A sensitive and specific serologic test for detection of Campylobacter pylori infection.

Authors:  D J Evans; D G Evans; D Y Graham; P D Klein
Journal:  Gastroenterology       Date:  1989-04       Impact factor: 22.682

Review 9.  Campylobacter pylori and peptic ulcer disease.

Authors:  D Y Graham
Journal:  Gastroenterology       Date:  1989-02       Impact factor: 22.682

10.  Immunoblot analysis of immune response to Campylobacter pylori and its clinical associations.

Authors:  H von Wulffen; H J Grote; S Gatermann; T Löning; B Berger; C Buhl
Journal:  J Clin Pathol       Date:  1988-06       Impact factor: 3.411
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  8 in total

1.  Serological discrimination of dogs infected with gastric Helicobacter spp. and uninfected dogs.

Authors:  D Strauss-Ayali; K W Simpson; A H Schein; P L McDonough; R H Jacobson; B A Valentine; J Peacock
Journal:  J Clin Microbiol       Date:  1999-05       Impact factor: 5.948

2.  Serology and urea breath test in the diagnosis of H. pylori infection.

Authors:  P R Hawtin
Journal:  Mol Biotechnol       Date:  1999-02       Impact factor: 2.695

3.  Serum antibody against Helicobacter pylori assayed by a new capture ELISA.

Authors:  H Nakata; H Itoh; Y Yokoya; J Kawai; S Nishioka; K Miyamoto; N Kitamoto; H Miyamoto; T Tanaka
Journal:  J Gastroenterol       Date:  1995-06       Impact factor: 7.527

4.  Serodiagnosis of Helicobacter pylori: comparison of enzyme-linked immunosorbent assays.

Authors:  N J Talley; D G Newell; J E Ormand; H A Carpenter; W R Wilson; A R Zinsmeister; G I Perez-Perez; M J Blaser
Journal:  J Clin Microbiol       Date:  1991-08       Impact factor: 5.948

5.  Evaluation of a commercial latex test for serological diagnosis of Helicobacter pylori infection in treated and untreated patients.

Authors:  A M Hirschl; M M Hirschl; J Berger; M L Rotter
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1991-11       Impact factor: 3.267

6.  Serological detection of Helicobacter pylori by a flow microsphere immunofluorescence assay.

Authors:  L M Best; S J Veldhuyzen van Zanten; G S Bezanson; D J Haldane; D A Malatjalian
Journal:  J Clin Microbiol       Date:  1992-09       Impact factor: 5.948

Review 7.  Blood tests in the management of Helicobacter pylori infection. Italian Helicobacter pylori Study Group.

Authors:  D Vaira; J Holton; M Menegatti; F Landi; C Ricci; A Ali; L Gatta; S Farinelli; C Acciardi; B Massardi; M Miglioli
Journal:  Gut       Date:  1998-07       Impact factor: 23.059

8.  Helicobacter pylori infection: relation with cardiovascular risk factors, ischaemic heart disease, and social class.

Authors:  L J Murray; K B Bamford; D P O'Reilly; E E McCrum; A E Evans
Journal:  Br Heart J       Date:  1995-11
  8 in total

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