CONTEXT: Recent secretome analyses suggest that human fat cells secrete hundreds of proteins (adipokines). OBJECTIVE: We made an overall analysis of their potential functional importance. MATERIALS AND METHODS: A secretome of 347 adipokines was evaluated by in silico analysis of their expression during adipocyte differentiation, regulation by obesity and adipose region. The gene expression in human adipose tissue was investigated in microarray studies using samples from different adipose depots from lean or obese patients. RESULTS: 60% of the adipokines were regulated by obesity and 50% between visceral and subcutaneous adipose region. Eight adipokines, all novel, scored particularly high in the in silico analysis. Among those, four were both regulated by obesity and adipose region, namely WNT1-inducible-signaling pathway protein 2, transmembrane glycoprotein NMB, inter-alpha-trypsin inhibitor heavy chain H5, and complement C4-A. Furthermore, many adipokines were extracellular matrix proteins. CONCLUSION: Several novel adipokines have potential important functional features warranting in depth analysis.
CONTEXT: Recent secretome analyses suggest that human fat cells secrete hundreds of proteins (adipokines). OBJECTIVE: We made an overall analysis of their potential functional importance. MATERIALS AND METHODS: A secretome of 347 adipokines was evaluated by in silico analysis of their expression during adipocyte differentiation, regulation by obesity and adipose region. The gene expression in human adipose tissue was investigated in microarray studies using samples from different adipose depots from lean or obesepatients. RESULTS: 60% of the adipokines were regulated by obesity and 50% between visceral and subcutaneous adipose region. Eight adipokines, all novel, scored particularly high in the in silico analysis. Among those, four were both regulated by obesity and adipose region, namely WNT1-inducible-signaling pathway protein 2, transmembrane glycoprotein NMB, inter-alpha-trypsin inhibitor heavy chain H5, and complement C4-A. Furthermore, many adipokines were extracellular matrix proteins. CONCLUSION: Several novel adipokines have potential important functional features warranting in depth analysis.
Authors: Tina Hörbelt; Christopher Tacke; Mariya Markova; Daniella Herzfeld de Wiza; Frederique Van de Velde; Marlies Bekaert; Yves Van Nieuwenhove; Silke Hornemann; Maria Rödiger; Nicole Seebeck; Elisabeth Friedl; Wenke Jonas; G Hege Thoresen; Oliver Kuss; Anke Rosenthal; Volker Lange; Andreas F H Pfeiffer; Annette Schürmann; Bruno Lapauw; Natalia Rudovich; Olga Pivovarova; D Margriet Ouwens Journal: Diabetologia Date: 2018-05-12 Impact factor: 10.122