PREMISE OF THE STUDY: A set of novel chloroplast microsatellite markers (cpSSRs) was developed for the bioenergy crop Miscanthus, and their utility in cross-species amplification was evaluated. METHODS AND RESULTS: Twenty-eight novel primers flanking cpSSR loci were designed from a complete chloroplast genome sequence of Saccharum officinarum, a species closely related to Miscanthus. These primers were then tested on eight Miscanthus species, among which 16 cpSSR loci were found to be polymorphic. The number of alleles per polymorphic locus ranged from two to seven, with an average 3.94 alleles. CONCLUSIONS: These cpSSR markers can be applied to all Miscanthus species and will be useful for studying Miscanthus population structure, diversity, and phylogeography.
PREMISE OF THE STUDY: A set of novel chloroplast microsatellite markers (cpSSRs) was developed for the bioenergy crop Miscanthus, and their utility in cross-species amplification was evaluated. METHODS AND RESULTS: Twenty-eight novel primers flanking cpSSR loci were designed from a complete chloroplast genome sequence of Saccharum officinarum, a species closely related to Miscanthus. These primers were then tested on eight Miscanthus species, among which 16 cpSSR loci were found to be polymorphic. The number of alleles per polymorphic locus ranged from two to seven, with an average 3.94 alleles. CONCLUSIONS: These cpSSR markers can be applied to all Miscanthus species and will be useful for studying Miscanthus population structure, diversity, and phylogeography.
Authors: Karine E Janner de Freitas; Carlos Busanello; Vívian Ebeling Viana; Camila Pegoraro; Filipe de Carvalho Victoria; Luciano Carlos da Maia; Antonio Costa de Oliveira Journal: Funct Integr Genomics Date: 2021-11-09 Impact factor: 3.410
Authors: Lindsay V Clark; Joe E Brummer; Katarzyna Głowacka; Megan C Hall; Kweon Heo; Junhua Peng; Toshihiko Yamada; Ji Hye Yoo; Chang Yeon Yu; Hua Zhao; Stephen P Long; Erik J Sacks Journal: Ann Bot Date: 2014-06-10 Impact factor: 4.357
Authors: Lindsay V Clark; Xiaoli Jin; Karen Koefoed Petersen; Kossanou G Anzoua; Larissa Bagmet; Pavel Chebukin; Martin Deuter; Elena Dzyubenko; Nicolay Dzyubenko; Kweon Heo; Douglas A Johnson; Uffe Jørgensen; Jens Bonderup Kjeldsen; Hironori Nagano; Junhua Peng; Andrey Sabitov; Toshihiko Yamada; Ji Hye Yoo; Chang Yeon Yu; Stephen P Long; Erik J Sacks Journal: Ann Bot Date: 2019-10-29 Impact factor: 4.357