Literature DB >> 2261433

Structure of the rat gene encoding cholesterol 7 alpha-hydroxylase.

D F Jelinek1, D W Russell.   

Abstract

Cholesterol 7 alpha-hydroxylase (7 alpha-hydroxylase) is a microsomal cytochrome P-450 that catalyzes the first and rate-limiting step in bile acid biosynthesis, the major catabolic pathway in cholesterol homeostasis. The gene encoding the rat 7 alpha-hydroxylase has been isolated and characterized. Southern blotting experiments demonstrated that the gene is present in a single copy in the rat genome. DNA sequence analysis showed that the 7 alpha-hydroxylase gene is unique among the characterized cytochrome P-450s in that it contains only six exons. Nuclease S1 and primer-extension mapping experiments positioned the 5'-ends of the 7 alpha-hydroxylase mRNA approximately 20-25 nucleotides downstream of a consensus TATAAA sequence. RNA blotting experiments demonstrated the presence of multiple 7 alpha-hydroxylase mRNAs that differ in the lengths of their 3'-untranslated regions.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 2261433     DOI： 10.1021/bi00486a001

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  10 in total

1.  New nucleotide sequence data on the EMBL File Server.

Authors: 
Journal:  Nucleic Acids Res       Date:  1990-11-25       Impact factor: 16.971

2.  A TaqI RFLP for the human arylsulfatase A gene.

Authors:  R Herzog; K Holzmann; N Blin
Journal:  Nucleic Acids Res       Date:  1990-11-25       Impact factor: 16.971

3.  Lucky, times ten: A career in Texas science.

Authors:  David W Russell
Journal:  J Biol Chem       Date:  2018-12-07       Impact factor: 5.157

4.  The 3'-untranslated region of the mouse cholesterol 7alpha-hydroxylase mRNA contains elements responsive to post-transcriptional regulation by bile acids.

Authors:  L B Agellon; S K Cheema
Journal:  Biochem J       Date:  1997-12-01       Impact factor: 3.857

5.  Activation of the silent endogenous cholesterol-7-alpha-hydroxylase gene in rat hepatoma cells: a new complementation group having resistance to 25-hydroxycholesterol.

Authors:  J K Leighton; S Dueland; M S Straka; J Trawick; R A Davis
Journal:  Mol Cell Biol       Date:  1991-04       Impact factor: 4.272

6.  Ethanol-extracted soy protein isolate results in elevation of serum cholesterol in exogenously hypercholesterolemic rats.

Authors:  W Ni; S Yoshida; Y Tsuda; K Nagao; M Sato; K Imaizumi
Journal:  Lipids       Date:  1999-07       Impact factor: 1.880

Review 7.  Nuclear receptors in the multidrug resistance through the regulation of drug-metabolizing enzymes and drug transporters.

Authors:  Yakun Chen; Yong Tang; Changxiong Guo; Jiuhui Wang; Debasish Boral; Daotai Nie
Journal:  Biochem Pharmacol       Date:  2012-02-04       Impact factor: 5.858

Review 8.  Role of orphan nuclear receptors in the regulation of drug-metabolising enzymes.

Authors:  Hongbing Wang; Edward L LeCluyse
Journal:  Clin Pharmacokinet       Date:  2003       Impact factor: 6.447

9.  Dietary cholesterol increases transcription of the human cholesteryl ester transfer protein gene in transgenic mice. Dependence on natural flanking sequences.

Authors:  X C Jiang; L B Agellon; A Walsh; J L Breslow; A Tall
Journal:  J Clin Invest       Date:  1992-10       Impact factor: 14.808

10.  Cholesterol and bile acids regulate cholesterol 7 alpha-hydroxylase expression at the transcriptional level in culture and in transgenic mice.

Authors:  M I Ramirez; D Karaoglu; D Haro; C Barillas; R Bashirzadeh; G Gil
Journal:  Mol Cell Biol       Date:  1994-04       Impact factor: 4.272
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.