PURPOSE: In our previous study, we have found that the hypoxia-inducible factor 1α (HIF-1α) is upregulated in renal cell carcinoma (RCC) tissues compared with para-cancer normal tissues by 2-dimensional polyacrylamide gel electrophoresis. It was reported that hypoxic conditions were correlated with cancer stem cell generation and HIF-1α acted as a transcription regulator in nuclear HIF-1α expression. Therefore, in this study we investigate the relation between CD133 and nuclear HIF-1α expression levels in RCC tissues. METHODS: In this study 61 RCC tissues from the patients that treated with radical nephrectomy were collected. Then, we investigated the expression of CD133 and nuclear HIF-1α expression by immunohistochemistry. To verify the relation between CD133 and nuclear HIF-1α expression, we treated 786-O cells with cobalt chloride. The expression of CD133 on 786-O cells was analyzed by flowcytometry. RESULTS: The immunohistochemical study showed that CD133 was correlated with tumor stage and metastatic stage, whereas nuclear HIF-1α had no association with clinicopathological parameters. However, the expression of nuclear HIF-1α was correlated with CD133. The CD133 expression in 786-O cells was enhanced by cobalt chloride, which meant that CD133 expression was affected by hypoxia. CONCLUSIONS: Our study showed that in RCC, CD133 expression was strongly related to nuclear HIF-1α and the expression of CD133 might be upregulated under hypoxia environment.
PURPOSE: In our previous study, we have found that the hypoxia-inducible factor 1α (HIF-1α) is upregulated in renal cell carcinoma (RCC) tissues compared with para-cancer normal tissues by 2-dimensional polyacrylamide gel electrophoresis. It was reported that hypoxic conditions were correlated with cancer stem cell generation and HIF-1α acted as a transcription regulator in nuclear HIF-1α expression. Therefore, in this study we investigate the relation between CD133 and nuclear HIF-1α expression levels in RCC tissues. METHODS: In this study 61 RCC tissues from the patients that treated with radical nephrectomy were collected. Then, we investigated the expression of CD133 and nuclear HIF-1α expression by immunohistochemistry. To verify the relation between CD133 and nuclear HIF-1α expression, we treated 786-O cells with cobalt chloride. The expression of CD133 on 786-O cells was analyzed by flowcytometry. RESULTS: The immunohistochemical study showed that CD133 was correlated with tumor stage and metastatic stage, whereas nuclear HIF-1α had no association with clinicopathological parameters. However, the expression of nuclear HIF-1α was correlated with CD133. The CD133 expression in 786-O cells was enhanced by cobalt chloride, which meant that CD133 expression was affected by hypoxia. CONCLUSIONS: Our study showed that in RCC, CD133 expression was strongly related to nuclear HIF-1α and the expression of CD133 might be upregulated under hypoxia environment.
Authors: S Miraglia; W Godfrey; A H Yin; K Atkins; R Warnke; J T Holden; R A Bray; E K Waller; D W Buck Journal: Blood Date: 1997-12-15 Impact factor: 22.113
Authors: D Corbeil; K Röper; A Hellwig; M Tavian; S Miraglia; S M Watt; P J Simmons; B Peault; D W Buck; W B Huttner Journal: J Biol Chem Date: 2000-02-25 Impact factor: 5.157
Authors: Maria V Gustafsson; Xiaowei Zheng; Teresa Pereira; Katarina Gradin; Shaobo Jin; Johan Lundkvist; Jorge L Ruas; Lorenz Poellinger; Urban Lendahl; Maria Bondesson Journal: Dev Cell Date: 2005-11 Impact factor: 12.270
Authors: Rafia S Al-Lamki; Jun Wang; Jun Yang; Natalie Burrows; Patrick H Maxwell; Timothy Eisen; Anne Y Warren; Sakari Vanharanta; Simon Pacey; Peter Vandenabeele; Jordan S Pober; John R Bradley Journal: Oncotarget Date: 2016-04-26