Comparison of the sulfur hexafluoride tracer and respiration chamber techniques for estimating methane emissions and correction for rectum methane output from dairy cows.

The objectives of the present study were to compare the sulfur hexafluoride (SF₆) and respiration chamber techniques for measuring methane (CH₄) emissions from dairy cows and to determine the proportion of CH₄ that is released through the rectum. Data used were derived from 20 early lactation dairy cows in a 2 × 2 factorial design study for 4 periods with 6 wk/period. The 4 treatment diets consisted of grass silage and 2 levels of concentrate (30 and 60% dry matter basis), with or without yeast supplement. At the end of each period, CH₄ emissions were measured simultaneously using the SF₆ and respiration chamber techniques when cows were housed in chambers. The SF₆ technique was also used when cows were housed in digestibility units (barn location) before and after respiratory chamber measurements (chamber location). The simultaneous measurements in chamber location revealed that CH₄ emission estimates by the SF₆ technique were similar to those by the respiration chamber technique in the first 3 periods, although the SF₆ estimates were significantly higher in period 4. The regression of all data from the 4 periods demonstrated a linear relationship between the SF₆ and respiration chamber measurements for total CH₄ emissions (g/d, R² = 0.69) and for CH₄ emissions per unit of milk yield (g/kg, R² = 0.88), and a quadratic relationship for CH₄ emissions per unit of dry matter intake (g/kg, R² = 0.64). The CH₄ emissions from the rectum were calculated as the difference between CH₄ estimates from the SF₆ technique when cows were housed in respiratory chambers and barn locations, which was 3% of the total CH₄ emissions from the mouth, nostrils, and rectum. The SF₆ estimates in the chamber location accounted for all sources of emissions, whereas those in the barn location, like that in grazing conditions, did not include CH₄ emission from the rectum. Therefore, the SF₆ measurements for grazing cattle should be adjusted for CH₄ emissions from the rectum (3% of total). We conclude that the SF₆ technique is reasonably accurate for estimating CH₄ emissions.