Literature DB >> 22609625

2b-RAD: a simple and flexible method for genome-wide genotyping.

Shi Wang1, Eli Meyer, John K McKay, Mikhail V Matz.   

Abstract

We describe 2b-RAD, a streamlined restriction site-associated DNA (RAD) genotyping method based on sequencing the uniform fragments produced by type IIB restriction endonucleases. Well-studied accessions of Arabidopsis thaliana were genotyped to validate the method's accuracy and to demonstrate fine-tuning of marker density as needed. The simplicity of the 2b-RAD protocol makes it particularly suitable for high-throughput genotyping as required for linkage mapping and profiling genetic variation in natural populations.

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Year:  2012        PMID： 22609625     DOI： 10.1038/nmeth.2023

Source DB:  PubMed          Journal:  Nat Methods        ISSN： 1548-7091            Impact factor:   28.547


  20 in total

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7.  Population genomics of parallel adaptation in threespine stickleback using sequenced RAD tags.

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8.  Common sequence polymorphisms shaping genetic diversity in Arabidopsis thaliana.

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  174 in total

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Review 5.  Harnessing the power of RADseq for ecological and evolutionary genomics.

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6.  A Genome-Wide Association Study Identifies the Genomic Region Associated with Shell Color in Yesso Scallop, Patinopecten yessoensis.

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Review 7.  Targeted capture in evolutionary and ecological genomics.

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10.  High-resolution linkage and quantitative trait locus mapping aided by genome survey sequencing: building up an integrative genomic framework for a bivalve mollusc.

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