OBJECTIVES: The aim of the present study was to investigate an antimicrobial additive containing experimental resin composite with regards to its impact on biofilm formation under oral conditions. METHODS: Biofilms were established in situ on composite specimens (n=192) which contained octenidine dihydrochloride (ODH, 3 wt.% or 6 wt.%). Samples without antimicrobial additive served as control (n=96). Composite specimens were fixed on custom made splints and exposed to the oral cavity of six healthy volunteers for three or seven days. Biofilm formation was assessed by scanning electron microscopy (SEM) and fluorescence microscopy (FM). RESULTS: The biofilm formation was significantly reduced on ODH containing samples compared to controls after three as well as after seven days in situ. FM evaluation additionally showed a lower viability of the reduced biofilms for both ODH concentrations. SIGNIFICANCE: During this short term investigation, incorporation of ODH into resin based composite materials caused biofilm inhibiting effects in situ.
OBJECTIVES: The aim of the present study was to investigate an antimicrobial additive containing experimental resin composite with regards to its impact on biofilm formation under oral conditions. METHODS: Biofilms were established in situ on composite specimens (n=192) which contained octenidine dihydrochloride (ODH, 3 wt.% or 6 wt.%). Samples without antimicrobial additive served as control (n=96). Composite specimens were fixed on custom made splints and exposed to the oral cavity of six healthy volunteers for three or seven days. Biofilm formation was assessed by scanning electron microscopy (SEM) and fluorescence microscopy (FM). RESULTS: The biofilm formation was significantly reduced on ODH containing samples compared to controls after three as well as after seven days in situ. FM evaluation additionally showed a lower viability of the reduced biofilms for both ODH concentrations. SIGNIFICANCE: During this short term investigation, incorporation of ODH into resin based composite materials caused biofilm inhibiting effects in situ.