OBJECTIVES: To evaluate if the incorporation of antimicrobial compounds to chelating agents or the use of chelating agents with antimicrobial activity as 7% maleic acid and peracetic acid show similar disinfection ability in comparison to conventional irrigants as sodium hypochlorite or iodine potassium iodide against biofilms developed on dentin. MATERIALS AND METHODS: The total bio-volume of live cells, the ratio of live cells and the substratum coverage of dentin infected intra-orally and treated with the irrigant solutions: MTAD, Qmix, Smear Clear, 7% maleic acid, 2% iodine potassium iodide, 4% peracetic acid, 2.5% and 5.25% sodium hypochlorite was measured by using confocal microscopy and the live/dead technique. Five samples were used for each irrigant solution. RESULTS: Several endodontic irrigants containing antimicrobials as clorhexidine (Qmix), cetrimide (Smear Clear), maleic acid, iodine compounds or antibiotics (MTAD) lacked an effective antibiofilm activity when the dentin was infected intra-orally. The irrigant solutions 4% peracetic acid and 2.5-5.25% sodium hypochlorite decrease significantly the number of live bacteria in biofilms, providing also cleaner dentin surfaces (p < 0.05). CONCLUSIONS: Several chelating agents containing antimicrobials could not remove nor kill significantly biofilms developed on intra-orally infected dentin, with the exception of sodium hypochlorite and 4% peracetic acid. Dissolution ability is mandatory for an appropriate eradication of biofilms attached to dentin.
OBJECTIVES: To evaluate if the incorporation of antimicrobial compounds to chelating agents or the use of chelating agents with antimicrobial activity as 7% maleic acid and peracetic acid show similar disinfection ability in comparison to conventional irrigants as sodium hypochlorite or iodine potassium iodide against biofilms developed on dentin. MATERIALS AND METHODS: The total bio-volume of live cells, the ratio of live cells and the substratum coverage of dentin infected intra-orally and treated with the irrigant solutions: MTAD, Qmix, Smear Clear, 7% maleic acid, 2% iodine potassium iodide, 4% peracetic acid, 2.5% and 5.25% sodium hypochlorite was measured by using confocal microscopy and the live/dead technique. Five samples were used for each irrigant solution. RESULTS: Several endodontic irrigants containing antimicrobials as clorhexidine (Qmix), cetrimide (Smear Clear), maleic acid, iodine compounds or antibiotics (MTAD) lacked an effective antibiofilm activity when the dentin was infected intra-orally. The irrigant solutions 4% peracetic acid and 2.5-5.25% sodium hypochlorite decrease significantly the number of live bacteria in biofilms, providing also cleaner dentin surfaces (p < 0.05). CONCLUSIONS: Several chelating agents containing antimicrobials could not remove nor kill significantly biofilms developed on intra-orally infected dentin, with the exception of sodium hypochlorite and 4% peracetic acid. Dissolution ability is mandatory for an appropriate eradication of biofilms attached to dentin.
Authors: Aldo del Carpio-Perochena; Clovis Monteiro Bramante; Flaviana Bombarda de Andrade; Amanda G Alves Maliza; Bruno Cavalini Cavenago; Marina A Marciano; Pablo Amoroso-Silva; Marco Hungaro Duarte Journal: Clin Oral Investig Date: 2015-02-26 Impact factor: 3.573
Authors: Colin Eliot; John F Hatton; Gregory P Stewart; Charles F Hildebolt; M Jane Gillespie; James L Gutmann Journal: Odontology Date: 2013-01-19 Impact factor: 2.634
Authors: Christian Tennert; Katharina Feldmann; Edwina Haamann; Ali Al-Ahmad; Marie Follo; Karl-Thomas Wrbas; Elmar Hellwig; Markus J Altenburger Journal: BMC Oral Health Date: 2014-11-04 Impact factor: 2.757
Authors: Aldo Del Carpio-Perochena; Clovis Monteiro Bramante; Marco Hungaro Duarte; Flaviana Bombarda de Andrade; Marcia Zardin Graeff; Marina Marciano da Silva; Bruno Cavalini Cavenago; Samuel Lucas Fernandes Journal: J Dent Res Dent Clin Dent Prospects Date: 2015-12-30