Literature DB >> 2259890

Trial and error in producing ankylosing-spondylitis-selective antisera according to Andrew Geczy.

C J Beukelman1, H C Quarles van Ufford, F P van Bree, P C Aerts, C Nieuwenhoff, G Reerink, A van Leeuwen, H van Dijk.   

Abstract

Geczy found that rabbit sera raised against Klebsiella strain K43 cross-reacted with the cells from HLA-B27 positive patients with ankylosing spondylitis (AS). Other laboratories failed to reproduce these results. After a series of unsuccessful attempts, however, we managed to prepare one selective antiserum, using E. coli, isolated from a Dutch Bechterew patient, in offspring of rabbits Geczy sent us. Ever since we obtained irreproducible results only. This paper reports about the many attempts we have made to produce a discriminating antiserum for use in a combined vital stain and dye-exclusion assay.

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Year:  1990        PMID: 2259890     DOI: 10.3109/03009749009097061

Source DB:  PubMed          Journal:  Scand J Rheumatol Suppl        ISSN: 0301-3847


  5 in total

Review 1.  Spondyloarthritis and the microbiome: new insights from an ancient hypothesis.

Authors:  Julia Manasson; Jose U Scher
Journal:  Curr Rheumatol Rep       Date:  2015-02       Impact factor: 4.592

Review 2.  Clinical, bacteriological, and serological aspects of Klebsiella infections and their spondylarthropathic sequelae.

Authors:  H Sahly; R Podschun
Journal:  Clin Diagn Lab Immunol       Date:  1997-07

3.  Failure of the serological determination of HLA-B27 due to antigen masking in patients with ankylosing spondylitis.

Authors:  J Neumüller; M Fischer; R Eberl
Journal:  Rheumatol Int       Date:  1993       Impact factor: 2.631

4.  Time for a gut check: evidence for the hypothesis that HLA-B27 predisposes to ankylosing spondylitis by altering the microbiome.

Authors:  James T Rosenbaum; Michael P Davey
Journal:  Arthritis Rheum       Date:  2011-11

5.  Comparison of the antibody responses to the 77 Klebsiella capsular types in ankylosing spondylitis and various rheumatic diseases.

Authors:  H Sahly; J Kekow; R Podschun; M Schaff; W L Gross; U Ullmann
Journal:  Infect Immun       Date:  1994-11       Impact factor: 3.441

  5 in total

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