BACKGROUND: We studied whether the sinuses might be foci for Pseudomonas aeruginosa lung infection. METHODS: Endoscopic Sinus Surgery was performed in 78 CF patients; PFGE was used for bacterial genotyping. Material from sinuses and lungs were Gram-stained to detect biofilms. Immunoglobulins were measured in serum and saliva. RESULTS: When P. aeruginosa was cultured simultaneously from the sinuses and the lungs they were genetically identical in 38 of the 40 patients (95%). In the sinuses, P. aeruginosa formed biofilms with minimal cellular inflammation, probably because of a significantly higher local production of secretory IgA compared with IgG (p<0.001). CONCLUSIONS: We have shown that P. aeruginosa form biofilm in the sinuses, which constitute an important bacterial reservoir for subsequent lung infection. The high amount of IgA in the upper airways probably protects P. aeruginosa from the inflammatory immune system, and they can proceed unnoticed into a permanent infectious focus that cannot be eradicated with antibiotics.
BACKGROUND: We studied whether the sinuses might be foci for Pseudomonas aeruginosa lung infection. METHODS: Endoscopic Sinus Surgery was performed in 78 CFpatients; PFGE was used for bacterial genotyping. Material from sinuses and lungs were Gram-stained to detect biofilms. Immunoglobulins were measured in serum and saliva. RESULTS: When P. aeruginosa was cultured simultaneously from the sinuses and the lungs they were genetically identical in 38 of the 40 patients (95%). In the sinuses, P. aeruginosa formed biofilms with minimal cellular inflammation, probably because of a significantly higher local production of secretory IgA compared with IgG (p<0.001). CONCLUSIONS: We have shown that P. aeruginosa form biofilm in the sinuses, which constitute an important bacterial reservoir for subsequent lung infection. The high amount of IgA in the upper airways probably protects P. aeruginosa from the inflammatory immune system, and they can proceed unnoticed into a permanent infectious focus that cannot be eradicated with antibiotics.
Authors: Peter Karempelis; Emily Karp; Nathan Rubin; Ryan Hunter; Jordan Dunitz; Holly Boyer Journal: Int Forum Allergy Rhinol Date: 2019-12-19 Impact factor: 3.858
Authors: J Manos; H Hu; B R Rose; C E Wainwright; I B Zablotska; J Cheney; L Turnbull; C B Whitchurch; K Grimwood; C Harmer; S N Anuj; C Harbour Journal: Eur J Clin Microbiol Infect Dis Date: 2013-07-07 Impact factor: 3.267
Authors: Anna C Zemke; Seyed Mehdi Nouraie; John Moore; Jordan R Gaston; Nicholas R Rowan; Joseph M Pilewski; Jennifer M Bomberger; Stella E Lee Journal: Int Forum Allergy Rhinol Date: 2019-06-04 Impact factor: 3.858
Authors: Renan Marrichi Mauch; Claudio Lucio Rossi; Marcos Tadeu Nolasco da Silva; Talita Bianchi Aiello; José Dirceu Ribeiro; Antônio Fernando Ribeiro; Niels Høiby; Carlos Emilio Levy Journal: Med Microbiol Immunol Date: 2019-01-31 Impact factor: 3.402
Authors: Maria E Møller; Mikkel C Alanin; Christian Grønhøj; Kasper Aanæs; Niels Høiby; Christian von Buchwald Journal: Am J Rhinol Allergy Date: 2017-09-01 Impact factor: 2.467