BACKGROUND: The quantification of hepatitis-B surface antigen (HBsAg) is useful to identify inactive carriers in chronically HBV infected patients and to predict interferon treatment outcome. OBJECTIVE: To compare two quantitative HBsAg assays for the detection of HBsAg mutants expressed in vitro. STUDY DESIGN: HBsAg mutants (n=35) were expressed in HuH7 cells and the supernatants were tested in two different quantitative HBsAg assays (Architect, Abbott and Elecsys, Roche). RESULTS: All HBsAg mutants were detected by both assays, but in general the results of the Architect system were higher than those of the Elecsys system. The detection of HBsAg mutants in comparison to wild type was similar using both assays. However, HBsAg mutation T123A was under quantified by the Architect, whereas HBsAg mutations P142L, P142S and G145K yielded lower results in the Elecsys system. CONCLUSIONS: Even though HBsAg assays are optimised for the detection of HBsAg mutants, discrepant results were obtained for some HBsAg mutants in two quantitative HBsAg assays. These findings have to be considered when testing samples from one patient with two different quantitative HBsAg assays.
BACKGROUND: The quantification of hepatitis-B surface antigen (HBsAg) is useful to identify inactive carriers in chronically HBV infectedpatients and to predict interferon treatment outcome. OBJECTIVE: To compare two quantitative HBsAg assays for the detection of HBsAg mutants expressed in vitro. STUDY DESIGN: HBsAg mutants (n=35) were expressed in HuH7 cells and the supernatants were tested in two different quantitative HBsAg assays (Architect, Abbott and Elecsys, Roche). RESULTS: All HBsAg mutants were detected by both assays, but in general the results of the Architect system were higher than those of the Elecsys system. The detection of HBsAg mutants in comparison to wild type was similar using both assays. However, HBsAg mutation T123A was under quantified by the Architect, whereas HBsAg mutations P142L, P142S and G145K yielded lower results in the Elecsys system. CONCLUSIONS: Even though HBsAg assays are optimised for the detection of HBsAg mutants, discrepant results were obtained for some HBsAg mutants in two quantitative HBsAg assays. These findings have to be considered when testing samples from one patient with two different quantitative HBsAg assays.
Authors: Timothy Na Archampong; Ceejay L Boyce; Margaret Lartey; Kwamena W Sagoe; Adjoa Obo-Akwa; Ernest Kenu; Jason T Blackard; Awewura Kwara Journal: Antivir Ther Date: 2016-05-11
Authors: Kuan-Hui Xiang; Eleftherios Michailidis; Hai Ding; Ya-Qin Peng; Ming-Ze Su; Yao Li; Xue-En Liu; Viet Loan Dao Thi; Xian-Fang Wu; William M Schneider; Charles M Rice; Hui Zhuang; Tong Li Journal: J Hepatol Date: 2016-09-17 Impact factor: 25.083
Authors: Mikael Gencay; Kirsten Hübner; Peter Gohl; Anja Seffner; Michael Weizenegger; Dionysios Neofytos; Richard Batrla; Andreas Woeste; Hyon-Suk Kim; Gaston Westergaard; Christine Reinsch; Eva Brill; Pham Thi Thu Thuy; Bui Huu Hoang; Mark Sonderup; C Wendy Spearman; Stephan Pabinger; Jérémie Gautier; Giuseppina Brancaccio; Massimo Fasano; Teresa Santantonio; Giovanni B Gaeta; Markus Nauck; Wolfgang E Kaminski Journal: PLoS One Date: 2017-05-04 Impact factor: 3.240