| Literature DB >> 2259449 |
C Speciale1, H Q Wu, J B Gramsbergen, W A Turski, U Ungerstedt, R Schwarcz.
Abstract
Kynurenic acid (KYNA) production from its bioprecursor L-kynurenine (KYN) was assessed in vivo by intrastriatal microdialysis in freely moving rats. In the absence of KYN, the extracellular concentration of KYNA was below the limit of assay sensitivity (i.e. less than 8 pmol/30 microliters). In the presence of KYN (50-2000 microM), KYNA concentration in the dialysate increased continuously to reach steady-state levels after 2h of perfusion. Introduction of the unspecific transaminase inhibitor aminooxyacetic acid (AOAA) through the dialysis probe caused a progressive decrease of extracellular KYNA, which reached dose-dependent minimal levels within 2 h. One mM AOAA caused an almost complete depletion of KYNA in the dialysate. These data demonstrate that extracellular KYNA can be assessed by microdialysis and that AOAA can be used as a tool to examine the neurobiology of KYNA in awake, freely moving animals.Entities:
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Year: 1990 PMID： 2259449 DOI： 10.1016/0304-3940(90)90410-b
Source DB: PubMed Journal: Neurosci Lett ISSN： 0304-3940 Impact factor: 3.046