BACKGROUND: Orthotopic liver transplantation (OLT) models in rats have been investigated in many studies. The reconstruction of hepatic artery is required for reliable OLT and also requires advanced skills. METHODS: The hepatic artery reconstructions by a hand-suture technique and a new method using a micro T-tube were investigated in rats with a whole-liver syngeneic graft. Operative time and postoperative patency were compared between the hand-suture and micro T-tube techniques. RESULTS: Our technique using the micro T-tube shortened the operative time of recipient surgery compared with the hand-suture technique and prolonged the operative time for the donor. The patency ratio was maintained at 24h after OLT with hand suturing but was significantly reduced with the micro T-tube, which had a patency ratio of 0.83 only up to 6h after OLT. CONCLUSION: The micro T-tube technique may have potential usefulness in the rat OLT model but requires further modification.
BACKGROUND: Orthotopic liver transplantation (OLT) models in rats have been investigated in many studies. The reconstruction of hepatic artery is required for reliable OLT and also requires advanced skills. METHODS: The hepatic artery reconstructions by a hand-suture technique and a new method using a micro T-tube were investigated in rats with a whole-liver syngeneic graft. Operative time and postoperative patency were compared between the hand-suture and micro T-tube techniques. RESULTS: Our technique using the micro T-tube shortened the operative time of recipient surgery compared with the hand-suture technique and prolonged the operative time for the donor. The patency ratio was maintained at 24h after OLT with hand suturing but was significantly reduced with the micro T-tube, which had a patency ratio of 0.83 only up to 6h after OLT. CONCLUSION: The micro T-tube technique may have potential usefulness in the rat OLT model but requires further modification.
Authors: B Dippe; D Kreisel; H Petrowsky; O Richter; S Krueger; D von Heimburg; M Schneider; E Hanisch; H J Wenisch; A Encke Journal: Transpl Int Date: 1992 Impact factor: 3.782
Authors: Y Kumamoto; M Suematsu; M Shimazu; Y Kato; T Sano; N Makino; K I Hirano; M Naito; G Wakabayashi; Y Ishimura; M Kitajima Journal: Hepatology Date: 1999-12 Impact factor: 17.425
Authors: Gaku Nakazawa; Fumiyuki Otsuka; Masataka Nakano; Marc Vorpahl; Saami K Yazdani; Elena Ladich; Frank D Kolodgie; Aloke V Finn; Renu Virmani Journal: J Am Coll Cardiol Date: 2011-03-15 Impact factor: 24.094