STUDY DESIGN: The expression of netrin-1 and its deleted in colorectal cancer (DCC) receptor was investigated in human lumbar discs using immunohistochemistry. OBJECTIVE: To investigate the expression of netrin-1 and DCC receptor in human diseased and healthy lumbar intervertebral discs (IVDs) and to clarify the correlation between netrin-1 expression and the degree of neurovascular ingrowth. SUMMARY OF BACKGROUND DATA: Previous studies have shown neurovascular ingrowth into the inner regions of degenerated IVD and suggested that the ingrowth may contribute to discogenic low back pain. Netrin-1 is an axon guidance molecule that regulates axons seeking their final targets and has been identified as involved in various pathological conditions, so is its DCC receptor. However, the role of netrin-1 in diseased IVDs remains unknown. METHODS: Thirty-five diseased IVD specimens were collected from 34 patients with different lumbar diseases during posterior lumbar interbody fusion. Eight normal discs were obtained at autopsy as control. Using polyclonal or monoclonal antibody, the disc slides were immmunostained to detect the expression and distribution of netrin-1, the DCC, the neuronal marker (neurofilament), and the vascular endothelial cell marker (CD34). RESULTS: Netrin-1 and DCC immunopositive cells distributed substantially from the annulus fibrosus to the nucleus pulposus (NP), and the immunopositivity was detected in the disc cells, endothelial cells and granulation tissue cells in the diseased discs. The percentage of netrin-1 positive disc cells of the NP was more than that of the annulus fibrosus. The expression of netrin-1 and DCC was weak in the normal discs. A significant positive correlation between the percentage of netrin-1 immunopositive disc cells and neurovascular scores was found. CONCLUSION: The increased expression of netrin-1 and DCC in diseased IVDs compared with controls suggested that they might play an important role in the process of neurovascular ingrowth.
STUDY DESIGN: The expression of netrin-1 and its deleted in colorectal cancer (DCC) receptor was investigated in human lumbar discs using immunohistochemistry. OBJECTIVE: To investigate the expression of netrin-1 and DCC receptor in human diseased and healthy lumbar intervertebral discs (IVDs) and to clarify the correlation between netrin-1 expression and the degree of neurovascular ingrowth. SUMMARY OF BACKGROUND DATA: Previous studies have shown neurovascular ingrowth into the inner regions of degenerated IVD and suggested that the ingrowth may contribute to discogenic low back pain. Netrin-1 is an axon guidance molecule that regulates axons seeking their final targets and has been identified as involved in various pathological conditions, so is its DCC receptor. However, the role of netrin-1 in diseased IVDs remains unknown. METHODS: Thirty-five diseased IVD specimens were collected from 34 patients with different lumbar diseases during posterior lumbar interbody fusion. Eight normal discs were obtained at autopsy as control. Using polyclonal or monoclonal antibody, the disc slides were immmunostained to detect the expression and distribution of netrin-1, the DCC, the neuronal marker (neurofilament), and the vascular endothelial cell marker (CD34). RESULTS:Netrin-1 and DCC immunopositive cells distributed substantially from the annulus fibrosus to the nucleus pulposus (NP), and the immunopositivity was detected in the disc cells, endothelial cells and granulation tissue cells in the diseased discs. The percentage of netrin-1 positive disc cells of the NP was more than that of the annulus fibrosus. The expression of netrin-1 and DCC was weak in the normal discs. A significant positive correlation between the percentage of netrin-1 immunopositive disc cells and neurovascular scores was found. CONCLUSION: The increased expression of netrin-1 and DCC in diseased IVDs compared with controls suggested that they might play an important role in the process of neurovascular ingrowth.
Authors: Guus G H van den Akker; Lars M T Eijssen; Stephen M Richardson; Lodewijk W van Rhijn; Judith A Hoyland; Tim J M Welting; Jan Willem Voncken Journal: Cartilage Date: 2018-04-09 Impact factor: 4.634
Authors: Pradeep Suri; Melody R Palmer; Yakov A Tsepilov; Maxim B Freidin; Cindy G Boer; Michelle S Yau; Daniel S Evans; Andrea Gelemanovic; Traci M Bartz; Maria Nethander; Liubov Arbeeva; Lennart Karssen; Tuhina Neogi; Archie Campbell; Dan Mellstrom; Claes Ohlsson; Lynn M Marshall; Eric Orwoll; Andre Uitterlinden; Jerome I Rotter; Gordan Lauc; Bruce M Psaty; Magnus K Karlsson; Nancy E Lane; Gail P Jarvik; Ozren Polasek; Marc Hochberg; Joanne M Jordan; Joyce B J Van Meurs; Rebecca Jackson; Carrie M Nielson; Braxton D Mitchell; Blair H Smith; Caroline Hayward; Nicholas L Smith; Yurii S Aulchenko; Frances M K Williams Journal: PLoS Genet Date: 2018-09-27 Impact factor: 6.020
Authors: Marie Udnesseter Lie; Linda Margareth Pedersen; Ingrid Heuch; Bendik Winsvold; Johannes Gjerstad; Eivind Hasvik; Øystein Petter Nygaard; Margreth Grotle; Dagfinn Matre; John-Anker Zwart; Kristian Bernhard Nilsen Journal: Front Genet Date: 2022-01-24 Impact factor: 4.599