Literature DB >> 22588378

An injectable nucleus pulposus implant restores compressive range of motion in the ovine disc.

Neil R Malhotra1, Woojin M Han, Jesse Beckstein, Jordan Cloyd, Weiliam Chen, Dawn M Elliott.   

Abstract

STUDY
DESIGN: Investigation of injectable nucleus pulposus (NP) implant.
OBJECTIVE: To assess the ability of a recently developed injectable hydrogel implant to restore nondegenerative disc mechanics through support of NP functional mechanics. SUMMARY OF BACKGROUND DATA: Although surgical intervention for low back pain is effective for some patients, treated discs undergo altered biomechanics and adjacent levels are at increased risk for accelerated degeneration. One potential treatment as an alternative to surgery for degenerated disc includes the percutaneous delivery of agents to support NP functional mechanics. The implants are delivered in a minimally invasive fashion, potentially on an outpatient basis, and do not preclude later surgical options. One of the challenges in designing such implants includes the need to match key NP mechanical behavior and mimic the role of native nondegenerate NP in spinal motion.
METHODS: The oxidized hyaluronic acid gelatin implant material was prepared. In vitro mechanical testing was performed in mature ovine bone-disc-bone units in 3 stages: intact, discectomy, and implantation versus sham. Tested samples were cut axially for qualitative structural observations.
RESULTS: Discectomy increased axial range of motion (ROM) significantly compared with intact. Hydrogel implantation reduced ROM 17% (P < 0.05) compared with discectomy and returned ROM to intact levels (ROM intact 0.71 mm, discectomy 0.87 mm, postimplantation 0.72 mm). Although ROM for the hydrogel implant group was statistically unchanged compared with the intact disc, ROM for sham discs, which received a discectomy and no implant, was significantly increased compared with intact. The compression and tension stiffness were decreased with discectomy and remained unchanged for both implant and sham groups as expected because the annulus fibrosus was not repaired. Gross morphology images confirmed no ejection of NP implant.
CONCLUSION: An injectable implant that mimics nondegenerate NP has the potential to return motion segment ROM to normal subsequent to injury.

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Year:  2012        PMID: 22588378      PMCID: PMC3717581          DOI: 10.1097/BRS.0b013e31825cdfb7

Source DB:  PubMed          Journal:  Spine (Phila Pa 1976)        ISSN: 0362-2436            Impact factor:   3.468


  44 in total

1.  Effect of removing the nucleus pulposus on the deformation of the annulus fibrosus during compression of the intervertebral disc.

Authors:  J R Meakin; D W Hukins
Journal:  J Biomech       Date:  2000-05       Impact factor: 2.712

2.  Effects of chondroitinase ABC on intradiscal pressure in sheep: an in vivo study.

Authors:  M Sasaki; T Takahashi; K Miyahara
Journal:  Spine (Phila Pa 1976)       Date:  2001-03-01       Impact factor: 3.468

3.  The effect of partial removal of the nucleus pulposus from the intervertebral disc on the response of the human annulus fibrosus to compression.

Authors:  J R Meakin; T W Redpath; D W Hukins
Journal:  Clin Biomech (Bristol, Avon)       Date:  2001-02       Impact factor: 2.063

4.  Biomechanical and morphologic evaluation of a three-dimensional fabric sheep artificial intervertebral disc: in vitro and in vivo analysis.

Authors:  K Kadoya; Y Kotani; K Abumi; T Takada; N Shimamoto; Y Shikinami; T Kadosawa; K Kaneda
Journal:  Spine (Phila Pa 1976)       Date:  2001-07-15       Impact factor: 3.468

Review 5.  The use of a quadruped as an in vivo model for the study of the spine - biomechanical considerations.

Authors:  Theo H Smit
Journal:  Eur Spine J       Date:  2002-04       Impact factor: 3.134

6.  Effect of a prosthetic disc nucleus on the mobility and disc height of the L4-5 intervertebral disc postnucleotomy.

Authors:  H J Wilke; S Kavanagh; S Neller; C Haid; L E Claes
Journal:  J Neurosurg       Date:  2001-10       Impact factor: 5.115

7.  Effect of anular repair on the healing strength of the intervertebral disc: a sheep model.

Authors:  B D Ahlgren; W Lui; H N Herkowitz; M M Panjabi; J P Guiboux
Journal:  Spine (Phila Pa 1976)       Date:  2000-09-01       Impact factor: 3.468

8.  Self-crosslinkable hydrogels composed of partially oxidized hyaluronan and gelatin: in vitro and in vivo responses.

Authors:  Lihui Weng; Hui Pan; Weiliam Chen
Journal:  J Biomed Mater Res A       Date:  2008-05       Impact factor: 4.396

Review 9.  The pathophysiology of the intervertebral disc.

Authors:  S R Bibby; D A Jones; R B Lee; J Yu
Journal:  Joint Bone Spine       Date:  2001-12       Impact factor: 4.929

Review 10.  Sheep model in orthopedic research: a literature review.

Authors:  L Martini; M Fini; G Giavaresi; R Giardino
Journal:  Comp Med       Date:  2001-08       Impact factor: 0.982

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  23 in total

1.  The fabrication of cryogel scaffolds incorporated with poloxamer 407 for potential use in the regeneration of the nucleus pulposus.

Authors:  Nicholas A Temofeew; Katherine R Hixon; Sarah H McBride-Gagyi; Scott A Sell
Journal:  J Mater Sci Mater Med       Date:  2017-01-31       Impact factor: 3.896

2.  Screening of hyaluronic acid-poly(ethylene glycol) composite hydrogels to support intervertebral disc cell biosynthesis using artificial neural network analysis.

Authors:  Claire G Jeong; Aubrey T Francisco; Zhenbin Niu; Robert L Mancino; Stephen L Craig; Lori A Setton
Journal:  Acta Biomater       Date:  2014-05-21       Impact factor: 8.947

3.  Nucleotomy reduces the effects of cyclic compressive loading with unloaded recovery on human intervertebral discs.

Authors:  Brent L Showalter; Neil R Malhotra; Edward J Vresilovic; Dawn M Elliott
Journal:  J Biomech       Date:  2014-06-06       Impact factor: 2.712

4.  Translation of an injectable triple-interpenetrating-network hydrogel for intervertebral disc regeneration in a goat model.

Authors:  Sarah E Gullbrand; Thomas P Schaer; Prateek Agarwal; Justin R Bendigo; George R Dodge; Weiliam Chen; Dawn M Elliott; Robert L Mauck; Neil R Malhotra; Lachlan J Smith
Journal:  Acta Biomater       Date:  2017-07-19       Impact factor: 8.947

5.  In vitro characterization of a stem-cell-seeded triple-interpenetrating-network hydrogel for functional regeneration of the nucleus pulposus.

Authors:  Lachlan J Smith; Deborah J Gorth; Brent L Showalter; Joseph A Chiaro; Elizabeth E Beattie; Dawn M Elliott; Robert L Mauck; Weiliam Chen; Neil R Malhotra
Journal:  Tissue Eng Part A       Date:  2014-03-21       Impact factor: 3.845

6.  Phenotypic stability, matrix elaboration and functional maturation of nucleus pulposus cells encapsulated in photocrosslinkable hyaluronic acid hydrogels.

Authors:  Dong Hwa Kim; John T Martin; Dawn M Elliott; Lachlan J Smith; Robert L Mauck
Journal:  Acta Biomater       Date:  2014-10-29       Impact factor: 8.947

Review 7.  Biomaterials for intervertebral disc regeneration and repair.

Authors:  Robert D Bowles; Lori A Setton
Journal:  Biomaterials       Date:  2017-03-15       Impact factor: 12.479

8.  Developments in intervertebral disc disease research: pathophysiology, mechanobiology, and therapeutics.

Authors:  Kathryn T Weber; Timothy D Jacobsen; Robert Maidhof; Justin Virojanapa; Chris Overby; Ona Bloom; Shaheda Quraishi; Mitchell Levine; Nadeen O Chahine
Journal:  Curr Rev Musculoskelet Med       Date:  2015-03

9.  A large animal model that recapitulates the spectrum of human intervertebral disc degeneration.

Authors:  S E Gullbrand; N R Malhotra; T P Schaer; Z Zawacki; J T Martin; J R Bendigo; A H Milby; G R Dodge; E J Vresilovic; D M Elliott; R L Mauck; L J Smith
Journal:  Osteoarthritis Cartilage       Date:  2016-08-26       Impact factor: 6.576

10.  Fibrin-genipin adhesive hydrogel for annulus fibrosus repair: performance evaluation with large animal organ culture, in situ biomechanics, and in vivo degradation tests.

Authors:  M Likhitpanichkul; M Dreischarf; S Illien-Junger; B A Walter; T Nukaga; R G Long; D Sakai; A C Hecht; J C Iatridis
Journal:  Eur Cell Mater       Date:  2014-07-18       Impact factor: 3.942

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