Darius Kornetka1, Martin Trammer, Jochen Zange. 1. Institute of Aerospace Medicine, Deutsches Zentrum für Luft- und Raumfahrt, DLR eV, Linder Höhe, 51147 Cologne, Germany. darius.kornetka@dlr.de
Abstract
OBJECT: The nuclear magnetic resonance (NMR) mobile-universal-surface-explorer (MOUSE) was evaluated in a pilot study to determine its ability to detect physiological changes in human skin caused by physical or pharmacological interventions. MATERIALS AND METHODS: The left lower arm skin thicknesses of ten male subjects were measured five times using a Profile NMR-MOUSE(®) ((1)H, 19 MHz) before and after a venous occlusion manoeuvre. In five of the subjects, the T(2eff) relaxation times were derived from a bi-exponential fitting and were determined in the dermis and subcutis before and after applying a salve containing capsaicin. RESULTS: The dermis (including the epidermis) showed rather homogeneous signal amplitudes. The subcutis was characterised by higher and more variable amplitudes. The full-skin thickness values were affirmed by ultrasound imaging. The NMR profiles did not show significant skin swelling due to venous occlusion. In the dermis, capsaicin caused significant (p < 0.05) decreases in both components of T(2eff) (100 ± 19 ms-19 ± 10 ms; 9.5 ± 0.5 ms-7.2 ± 1.6 ms). In the subcutis, the T(2eff) was not affected. CONCLUSION: In principle, NMR-MOUSE profiles are capable of detecting skin structure. However, precise measurements are jeopardised by poor reproducibility, long acquisition times, and incompatibility between the geometries of the sensitive area of the instrument and the non-planar structure of the skin. In the dermis, T(2eff) contrast could be used to detect the changes in tissue composition caused by inflammatory reactions.
OBJECT: The nuclear magnetic resonance (NMR) mobile-universal-surface-explorer (MOUSE) was evaluated in a pilot study to determine its ability to detect physiological changes in human skin caused by physical or pharmacological interventions. MATERIALS AND METHODS: The left lower arm skin thicknesses of ten male subjects were measured five times using a Profile NMR-MOUSE(®) ((1)H, 19 MHz) before and after a venous occlusion manoeuvre. In five of the subjects, the T(2eff) relaxation times were derived from a bi-exponential fitting and were determined in the dermis and subcutis before and after applying a salve containing capsaicin. RESULTS: The dermis (including the epidermis) showed rather homogeneous signal amplitudes. The subcutis was characterised by higher and more variable amplitudes. The full-skin thickness values were affirmed by ultrasound imaging. The NMR profiles did not show significant skin swelling due to venous occlusion. In the dermis, capsaicin caused significant (p < 0.05) decreases in both components of T(2eff) (100 ± 19 ms-19 ± 10 ms; 9.5 ± 0.5 ms-7.2 ± 1.6 ms). In the subcutis, the T(2eff) was not affected. CONCLUSION: In principle, NMR-MOUSE profiles are capable of detecting skin structure. However, precise measurements are jeopardised by poor reproducibility, long acquisition times, and incompatibility between the geometries of the sensitive area of the instrument and the non-planar structure of the skin. In the dermis, T(2eff) contrast could be used to detect the changes in tissue composition caused by inflammatory reactions.
Authors: S Diridollou; A Maillet; A Pavy-Le Traon; F Louisy; D Black; M Berson; J M Grégoire; Y Gall; P Arbeille Journal: Eur J Ultrasound Date: 2001-07
Authors: S Diridollou; A Pavy-Le Traon; A Maillet; F Bellossi; D Black; F Patat; J M Lagarde; M Berson; Y Gall Journal: Skin Res Technol Date: 2000-08 Impact factor: 2.365
Authors: B J Van der Schueren; J N de Hoon; F H Vanmolkot; A Van Hecken; M Depre; S A Kane; I De Lepeleire; S R Sinclair Journal: Br J Clin Pharmacol Date: 2007-06-19 Impact factor: 4.335
Authors: Agnes Machnik; Wolfgang Neuhofer; Jonathan Jantsch; Anke Dahlmann; Tuomas Tammela; Katharina Machura; Joon-Keun Park; Franz-Xaver Beck; Dominik N Müller; Wolfgang Derer; Jennifer Goss; Agata Ziomber; Peter Dietsch; Hubertus Wagner; Nico van Rooijen; Armin Kurtz; Karl F Hilgers; Kari Alitalo; Kai-Uwe Eckardt; Friedrich C Luft; Dontscho Kerjaschki; Jens Titze Journal: Nat Med Date: 2009-05-03 Impact factor: 53.440