Literature DB >> 22576266

Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale.

Steven P Hanlon1, Andrea Camattari, Sandra Abad, Anton Glieder, Matthias Kittelmann, Stephan Lütz, Beat Wirz, Margit Winkler.   

Abstract

A panel of human flavin monooxygenases were heterologously expressed in E. coli to obtain ready-to-use biocatalysts for the in vitro preparation of human drug metabolites. Moclobemide-N-oxide (65 mg) was the first high-priced metabolite prepared with recombinant hFMO3 on the multi-milligram scale.

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Year:  2012        PMID： 22576266     DOI： 10.1039/c2cc17878h

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  5 in total

1.  Human FMO2-based microbial whole-cell catalysts for drug metabolite synthesis.

Authors:  Martina Geier; Thorsten Bachler; Steven P Hanlon; Fabian K Eggimann; Matthias Kittelmann; Hansjörg Weber; Stephan Lütz; Beat Wirz; Margit Winkler
Journal:  Microb Cell Fact       Date:  2015-06-12       Impact factor: 5.328

2.  Predicting the Metabolic Sites by Flavin-Containing Monooxygenase on Drug Molecules Using SVM Classification on Computed Quantum Mechanics and Circular Fingerprints Molecular Descriptors.

Authors:  Chien-Wei Fu; Thy-Hou Lin
Journal:  PLoS One       Date:  2017-01-10       Impact factor: 3.240

Review 3.  Flavin-dependent N-hydroxylating enzymes: distribution and application.

Authors:  Carolin Mügge; Thomas Heine; Alvaro Gomez Baraibar; Willem J H van Berkel; Caroline E Paul; Dirk Tischler
Journal:  Appl Microbiol Biotechnol       Date:  2020-06-05       Impact factor: 4.813

Review 4.  Human Enzymes for Organic Synthesis.

Authors:  Margit Winkler; Martina Geier; Steven P Hanlon; Bernd Nidetzky; Anton Glieder
Journal:  Angew Chem Int Ed Engl       Date:  2018-09-11       Impact factor: 15.336

5.  Production of drug metabolites by human FMO3 in Escherichia coli.

Authors:  Gianluca Catucci; Gianfranco Gilardi; Sheila J Sadeghi
Journal:  Microb Cell Fact       Date:  2020-03-20       Impact factor: 5.328
  5 in total

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