| Literature DB >> 22574182 |
Tamar Sztal1, Henry Chung, Silke Berger, Peter D Currie, Philip Batterham, Phillip J Daborn.
Abstract
The sequencing of numerous insect genomes has revealed dynamic changes in the number and identity of cytochrome P450 genes in different insects. In the evolutionary sense, the rapid birth and death of many P450 genes is observed, with only a small number of P450 genes showing orthology between insects with sequenced genomes. It is likely that these conserved P450s function in conserved pathways. In this study, we demonstrate the P450 gene, Cyp301a1, present in all insect genomes sequenced to date, affects the formation of the adult cuticle in Drosophila melanogaster. A Cyp301a1 piggyBac insertion mutant and RNAi of Cyp301a1 both show a similar cuticle malformation phenotype, which can be reduced by 20-hydroxyecdysone, suggesting that Cyp301a1 is an important gene involved in the formation of the adult cuticle and may be involved in ecdysone regulation in this tissue.Entities:
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Year: 2012 PMID： 22574182 PMCID： PMC3344891 DOI： 10.1371/journal.pone.0036544
Source DB: PubMed Journal: PLoS One ISSN： 1932-6203 Impact factor: 3.240