BACKGROUND AND AIMS: A liquid chromatography coupled with mass spectrometry based metabonomics approach was applied to investigate the protective effects of rosiglitazone (RGTZ) and caloric restriction (CR) for renal senescence in a rat model. METHODS: Kidney tissues and serum samples were collected from four groups of rats, including 12- month and 24-month ad libitum fed rats, RGTZ and CR treated 24-month rats. Multivariate data analysis was performed on the mass data of metabonomic profiles to detect the differences among the groups. RESULTS: By metabolite profiling and partial least squares discriminate analysis, 23 renal senescence-related endogenous metabolites were discovered, including phospholipids, carnitine, acetylcarnitine, and creatinine, most of which were related to the oxidative stress and lipid metabolism. CONCLUSION: Renal senescence is characterized by oxidative stress and changes in lipid metabolism, and RGTZ administration and CR treatment may have similar protective effects for renal senescence via restraining oxidative stress and lipid metabolism.
BACKGROUND AND AIMS: A liquid chromatography coupled with mass spectrometry based metabonomics approach was applied to investigate the protective effects of rosiglitazone (RGTZ) and caloric restriction (CR) for renal senescence in a rat model. METHODS: Kidney tissues and serum samples were collected from four groups of rats, including 12- month and 24-month ad libitum fed rats, RGTZ and CR treated 24-month rats. Multivariate data analysis was performed on the mass data of metabonomic profiles to detect the differences among the groups. RESULTS: By metabolite profiling and partial least squares discriminate analysis, 23 renal senescence-related endogenous metabolites were discovered, including phospholipids, carnitine, acetylcarnitine, and creatinine, most of which were related to the oxidative stress and lipid metabolism. CONCLUSION: Renal senescence is characterized by oxidative stress and changes in lipid metabolism, and RGTZ administration and CR treatment may have similar protective effects for renal senescence via restraining oxidative stress and lipid metabolism.