Emina Malisic1, Radmila Jankovic, Ksenija Jakovljevic. 1. Laboratory for Molecular Genetics, Institute for Oncology and Radiology of Serbia, Pasterova 14, 11 000, Belgrade, Serbia. eminamalisic@yahoo.co.uk
Abstract
PURPOSE: The objective of this study was to investigate the presence of human papillomavirus (HPV) infection in ovarian carcinoma samples from Serbian women and correlate them with clinicopathological characteristics of disease and patients' characteristics. METHODS: Fifty-four ovarian carcinoma patients were included in the study. DNA was isolated by salting out method from tumor tissue obtained after surgical treatment. Presence of HPV infection was detected through polymerase chain reaction amplification of a 150-bp fragment of L1 viral gene by GP5+/GP6+ primers. HPV genotyping was performed by DNA sequencing. RESULTS: HPV DNA was present in 4/54 (7.4 %) ovarian carcinomas. All HPV-positive tumors contained high-risk HPV16 type. HPV infection was more common in advanced opposite to localized disease. The median age of diagnosis of disease varied from 57 years for patients with HPV infection to 59 years for patients without HPV infection. CONCLUSIONS: Our results indicate that HPV infection may play a limited role in ovarian carcinogenesis.
PURPOSE: The objective of this study was to investigate the presence of humanpapillomavirus (HPV) infection in ovarian carcinoma samples from Serbian women and correlate them with clinicopathological characteristics of disease and patients' characteristics. METHODS: Fifty-four ovarian carcinomapatients were included in the study. DNA was isolated by salting out method from tumor tissue obtained after surgical treatment. Presence of HPV infection was detected through polymerase chain reaction amplification of a 150-bp fragment of L1 viral gene by GP5+/GP6+ primers. HPV genotyping was performed by DNA sequencing. RESULTS:HPV DNA was present in 4/54 (7.4 %) ovarian carcinomas. All HPV-positive tumors contained high-risk HPV16 type. HPV infection was more common in advanced opposite to localized disease. The median age of diagnosis of disease varied from 57 years for patients with HPV infection to 59 years for patients without HPV infection. CONCLUSIONS: Our results indicate that HPV infection may play a limited role in ovarian carcinogenesis.
Authors: Othman A Al-Shabanah; Mohamed M Hafez; Zeinab K Hassan; Mohamed M Sayed-Ahmed; Waleed N Abozeed; Salem S Al-Rejaie; Abdulmalik A Alsheikh Journal: Virol J Date: 2013-11-20 Impact factor: 4.099
Authors: Sagarika Banerjee; Tian Tian; Zhi Wei; Natalie Shih; Michael D Feldman; James C Alwine; George Coukos; Erle S Robertson Journal: Oncotarget Date: 2017-05-30