Literature DB >> 22567913

[Study of phototrophic purple bacterium Rhodobacter sphaeroides cell morphology of wild-type and ipt-transformant by atomic force and electron microscopy].

A V Machulin, L D Smolygina, N E Suzina, O P Serdiuk.   

Abstract

A comparative study of phototrophic purple bacterium Rhodobacter sphaeroides cell morphology of wild-type and ipt-transformant was done by atomic force and electron microscopy. It was shown that transformation led to a decrease in the number or total disappearance of the flagella, as well as to changes in the structure of the outer membrane of the bacteria cell wall. On the wild-type cell surface phage-like structures were found, and in transformed cells at their places hollows were identified. This study significantly extends an understanding of the changes occurring in the ipt-transformants of phototrophic purple bacterium Rhodobacter sphaeroides. This investigation not only confirmed earlier obtained data about the differences in the wild-type and ipt-transformant phototrophic purple bacteria cell wall, but also showed fine changes in the structure of its outer membrane.

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Year:  2012        PMID: 22567913

Source DB:  PubMed          Journal:  Biofizika        ISSN: 0006-3029


  1 in total

1.  Direct transition of pGA482:ipt plasmid bearing the cytokinin biosynthesis gene into the cells of phototrophic purple bacteria Rhodobacter sphaeroides and Rhodopseudomonas palustris by electroporation.

Authors:  O P Serdyuk; L D Smolygina; E M Chekunova; E P Sannikova; G N Shirshikova; A N Khusnutdinova; N V Yartseva
Journal:  Dokl Biochem Biophys       Date:  2013-08-23       Impact factor: 0.788

  1 in total

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