BACKGROUND: Histological chorioamnionitis (CHORIO) may increase inflammatory mediators in the lungs of preterm infants. OBJECTIVE: To study the impact of CHORIO on tracheal aspirate (TA) cytokines in ventilated infants. DESIGN/ METHODS: TA samples collected within 48 hours after birth from 40 ventilated neonates (gestational age [GA] <30 weeks, body weight [BW] <1250 g) were analyzed. Levels of 12 cytokines (interleukin [IL]-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, epidermal growth factor [EGF], interferon-γ [IFN-γ], monocyte chemotactic protein-1 [MCP-1], tumor necrosis factor-α [TNF-α], vascular endothelial growth factor [VEGF]) were measured using a biochip multianalyte immunoassay (Randox Laboratories, Antrim, UK). Total protein was measured by the Bradford assay. CHORIO assessment was done by a blinded pathologist. RESULTS: Twenty-six infants (GA 26.6 ± 1.4 weeks, BW 852 ± 162 g) had no CHORIO and 14 (GA 25.1 ± 1.0 weeks, BW 776 ± 164 g) had CHORIO. IL-1α, IL-1β, IL-8, and VEGF were significantly higher in TA of infants with CHORIO. After correction for dilution, IL-1α, IL-1β, and IL-8 were significantly elevated. Increased TA total cell count correlated with CHORIO, VEGF, EGF, MCP-1, IL-8, and IL-6 TA levels (all p ≤ 0.02). Ventilator, oxygen supplementation, and hospital days correlated with TA IFN-γ levels (all p ≤ 0.01). CONCLUSION: CHORIO is associated with increased specific proinflammatory mediators in TA samples of preterm infants.
BACKGROUND: Histological chorioamnionitis (CHORIO) may increase inflammatory mediators in the lungs of preterm infants. OBJECTIVE: To study the impact of CHORIO on tracheal aspirate (TA) cytokines in ventilated infants. DESIGN/ METHODS: TA samples collected within 48 hours after birth from 40 ventilated neonates (gestational age [GA] <30 weeks, body weight [BW] <1250 g) were analyzed. Levels of 12 cytokines (interleukin [IL]-1α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, epidermal growth factor [EGF], interferon-γ [IFN-γ], monocyte chemotactic protein-1 [MCP-1], tumor necrosis factor-α [TNF-α], vascular endothelial growth factor [VEGF]) were measured using a biochip multianalyte immunoassay (Randox Laboratories, Antrim, UK). Total protein was measured by the Bradford assay. CHORIO assessment was done by a blinded pathologist. RESULTS: Twenty-six infants (GA 26.6 ± 1.4 weeks, BW 852 ± 162 g) had no CHORIO and 14 (GA 25.1 ± 1.0 weeks, BW 776 ± 164 g) had CHORIO. IL-1α, IL-1β, IL-8, and VEGF were significantly higher in TA of infants with CHORIO. After correction for dilution, IL-1α, IL-1β, and IL-8 were significantly elevated. Increased TA total cell count correlated with CHORIO, VEGF, EGF, MCP-1, IL-8, and IL-6 TA levels (all p ≤ 0.02). Ventilator, oxygen supplementation, and hospital days correlated with TA IFN-γ levels (all p ≤ 0.01). CONCLUSION: CHORIO is associated with increased specific proinflammatory mediators in TA samples of preterm infants.
Authors: Ravi Misra; Syed Shah; Deborah Fowell; Hongyue Wang; Kristin Scheible; Sara Misra; Heidie Huyck; Claire Wyman; Rita M Ryan; Anne Marie Reynolds; Tom Mariani; Philip J Katzman; Gloria S Pryhuber Journal: Hum Immunol Date: 2015-03-20 Impact factor: 2.850
Authors: Brandie D Wagner; Marci K Sontag; J Kirk Harris; Joshua I Miller; Lindsey Morrow; Charles E Robertson; Mark J Stephens; Brenda B Poindexter; Steven H Abman; Peter M Mourani Journal: J Matern Fetal Neonatal Med Date: 2017-11-28
Authors: Huanxing Sun; Rayman Choo-Wing; Juan Fan; Lin Leng; Mansoor A Syed; Alissa A Hare; William L Jorgensen; Richard Bucala; Vineet Bhandari Journal: Respir Res Date: 2013-02-28