Literature DB >> 22566056

Whole mount immunohistochemistry and in situ hybridization of larval and adult zebrafish dental tissues.

Barbara Verstraeten1, Ellen Sanders, Ann Huysseune.   

Abstract

Tooth development is increasingly being studied in a variety of vertebrate model organisms, each contributing its own perspective to our understanding of dental diversity. In situ hybridization and immunohistochemistry are well-established and frequently used techniques to study the presence of mRNA and protein. Here, we describe a protocol for whole mount immunohistochemistry and in situ hybridization that can be applied to all stages of zebrafish development and dissected bony parts. The description of these protocols is followed by the outline of a quick decalcification method and the procedure for embedding in epoxy resin to obtain serial sections with high histological quality.

Entities:  

Mesh：

Year:  2012        PMID： 22566056     DOI： 10.1007/978-1-61779-860-3_16

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  11 in total

1.  Development of an in vitro cell system from zebrafish suitable to study bone cell differentiation and extracellular matrix mineralization.

Authors:  Parameswaran Vijayakumar; Vincent Laizé; João Cardeira; Marlene Trindade; M Leonor Cancela
Journal:  Zebrafish       Date:  2013-08-02       Impact factor: 1.985

Review 2.  Imaging the zebrafish dentition: from traditional approaches to emerging technologies.

Authors:  Bart Bruneel; Markus Mathä; Rik Paesen; Marcel Ameloot; Wolfgang J Weninger; Ann Huysseune
Journal:  Zebrafish       Date:  2015-01-05       Impact factor: 1.985

3.  Genetic Defects in TAPT1 Disrupt Ciliogenesis and Cause a Complex Lethal Osteochondrodysplasia.

Authors:  Sofie Symoens; Aileen M Barnes; Charlotte Gistelinck; Fransiska Malfait; Brecht Guillemyn; Wouter Steyaert; Delfien Syx; Sanne D'hondt; Martine Biervliet; Julie De Backer; Eckhard P Witten; Sergey Leikin; Elena Makareeva; Gabriele Gillessen-Kaesbach; Ann Huysseune; Kris Vleminckx; Andy Willaert; Anne De Paepe; Joan C Marini; Paul J Coucke
Journal:  Am J Hum Genet       Date:  2015-09-10       Impact factor: 11.025

4.  Wnt signaling during tooth replacement in zebrafish (Danio rerio): pitfalls and perspectives.

Authors:  Ann Huysseune; Mieke Soenens; Fien Elderweirdt
Journal:  Front Physiol       Date:  2014-10-06       Impact factor: 4.566

5.  Zebrafish models for ectopic mineralization disorders: practical issues from morpholino design to post-injection observations.

Authors:  Mohammad Jakir Hosen; Olivier M Vanakker; Andy Willaert; Ann Huysseune; Paul Coucke; Anne De Paepe
Journal:  Front Genet       Date:  2013-05-08       Impact factor: 4.599

6.  Distinct patterns of notochord mineralization in zebrafish coincide with the localization of Osteocalcin isoform 1 during early vertebral centra formation.

Authors:  Anabela Bensimon-Brito; João Cardeira; Maria Leonor Cancela; Ann Huysseune; Paul Eckhard Witten
Journal:  BMC Dev Biol       Date:  2012-10-09       Impact factor: 1.978

Review 7.  "Zebrafishing" for novel genes relevant to the glomerular filtration barrier.

Authors:  Nils Hanke; Lynne Staggs; Patricia Schroder; Jennifer Litteral; Susanne Fleig; Jessica Kaufeld; Cornelius Pauli; Hermann Haller; Mario Schiffer
Journal:  Biomed Res Int       Date:  2013-09-11       Impact factor: 3.411

8.  Beta-Catenin and Plakoglobin Expression during Zebrafish Tooth Development and Replacement.

Authors:  Barbara Verstraeten; Jolanda van Hengel; Ann Huysseune
Journal:  PLoS One       Date:  2016-03-03       Impact factor: 3.240

9.  Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin.

Authors:  C Gistelinck; R Gioia; A Gagliardi; F Tonelli; L Marchese; L Bianchi; C Landi; L Bini; A Huysseune; P E Witten; A Staes; K Gevaert; N De Rocker; B Menten; F Malfait; S Leikin; S Carra; R Tenni; A Rossi; A De Paepe; P Coucke; A Willaert; A Forlino
Journal:  Sci Rep       Date:  2016-02-15       Impact factor: 4.379

10.  Deep sequencing revealed molecular signature of horizontal gene transfer of plant like transcripts in the mosquito Anopheles culicifacies: an evolutionary puzzle.

Authors:  Punita Sharma; Tanwee Das De; Swati Sharma; Ashwani Kumar Mishra; Tina Thomas; Sonia Verma; Vandana Kumari; Suman Lata; Namita Singh; Neena Valecha; Kailash Chand Pandey; Rajnikant Dixit
Journal:  F1000Res       Date:  2015-12-30
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.