Literature DB >> 22566045

Detection of apoptosis by TUNEL assay.

Kateryna Kyrylkova1, Sergiy Kyryachenko, Mark Leid, Chrissa Kioussi.   

Abstract

Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay has been designed to detect apoptotic cells that undergo extensive DNA degradation during the late stages of apoptosis. The method is based on the ability of TdT to label blunt ends of double-stranded DNA breaks independent of a template. This chapter describes an assay for detection of apoptotic cells during mouse odontogenesis using a colorimetric TUNEL system.

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Year:  2012        PMID： 22566045     DOI： 10.1007/978-1-61779-860-3_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


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