OBJECTIVE AND DESIGN: The hypothesis that aspiration of gastric fluid drives the anti-ovalbumin response toward a Th2 reaction even in animals not prone to Th2 responses was evaluated. SUBJECTS: Forty-eight male C57BL/6 mice were used. METHODS: Mice were sensitized and challenged with ovalbumin starting 5 weeks prior to the initiation of weekly aspirations of either gastric fluid or normal saline as a control. Weekly aspiration continued during the course of exposure to ovalbumin. TREATMENT: Aspiration consisted of 50 μl of gastric fluid with 50 μl of 0.9 % normal saline used as a control. Antigen exposure consisted of sensitization to ovalbumin via intraperitoneal injection on days 0 and 14 and challenge on day 21 with aerosolized antigen for 30 min. RESULTS: No evidence of a shift toward a Th2 response as a result of gastric fluid aspiration was seen in the Th1-prone strain utilized, although a profound down-regulation of a broad array of T cell-associated cytokines and chemokines and up-regulation of macrophage-associated markers was observed as a result of aspiration. CONCLUSIONS: These data provide support for the hypothesis that the clinical association between asthma and gastroesophageal reflux disease (GERD) does not involve an exacerbation of asthma by GERD-associated aspiration of gastric fluid, but may cause immune reactions unrelated to the asthma pathology.
OBJECTIVE AND DESIGN: The hypothesis that aspiration of gastric fluid drives the anti-ovalbumin response toward a Th2 reaction even in animals not prone to Th2 responses was evaluated. SUBJECTS: Forty-eight male C57BL/6 mice were used. METHODS:Mice were sensitized and challenged with ovalbumin starting 5 weeks prior to the initiation of weekly aspirations of either gastric fluid or normal saline as a control. Weekly aspiration continued during the course of exposure to ovalbumin. TREATMENT: Aspiration consisted of 50 μl of gastric fluid with 50 μl of 0.9 % normal saline used as a control. Antigen exposure consisted of sensitization to ovalbumin via intraperitoneal injection on days 0 and 14 and challenge on day 21 with aerosolized antigen for 30 min. RESULTS: No evidence of a shift toward a Th2 response as a result of gastric fluid aspiration was seen in the Th1-prone strain utilized, although a profound down-regulation of a broad array of T cell-associated cytokines and chemokines and up-regulation of macrophage-associated markers was observed as a result of aspiration. CONCLUSIONS: These data provide support for the hypothesis that the clinical association between asthma and gastroesophageal reflux disease (GERD) does not involve an exacerbation of asthma by GERD-associated aspiration of gastric fluid, but may cause immune reactions unrelated to the asthma pathology.
Authors: Neerad C Mishra; Jules Rir-Sima-Ah; Raymond J Langley; Shashi P Singh; Juan C Peña-Philippides; Takeshi Koga; Seddigheh Razani-Boroujerdi; Julie Hutt; Matthew Campen; K Chul Kim; Yohannes Tesfaigzi; Mohan L Sopori Journal: J Immunol Date: 2008-06-01 Impact factor: 5.422
Authors: Chih Mei Cheng; Chong Chao Hsieh; Chang Sheng Lin; Zen Kong Dai; Pin Keng Shih; Mary Lou Everett; Anitra D Thomas; William Parker; R Duane Davis; Shu S Lin Journal: Immunobiology Date: 2009-05-23 Impact factor: 3.144
Authors: A S Barbas; T E Downing; K R Balsara; H E Tan; G J Rubinstein; Z E Holzknecht; B H Collins; W Parker; R D Davis; S S Lin Journal: Eur J Clin Invest Date: 2008-08 Impact factor: 4.686
Authors: Anitra D Thomas; Kuei-Ying Su; Jui-Chih Chang; Jason H Leung; Sean M Lee; Zoie E Holzknecht; Mary Lou Everett; William Parker; R Duane Davis; Shu S Lin Journal: Surg Endosc Date: 2009-11-14 Impact factor: 4.584