Maryam Moazeni1, Niloofar Rashidi1, Ahmad R Shahverdi2, Fatemeh Noorbakhsh3, Sassan Rezaie1,4. 1. Div. of Molecular Biology, Dep. of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. 2. Dep. of Pharmaceutical Biotechnology and Medical Nanotechnology zzm321990Research Center, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. 3. Dep. Of Biology, Islamic Azad University, Varamin-Pishva Branch, Varamin, Iran. 4. Dept. of Medical Biotechnology, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran.
Abstract
BACKGROUND: To develop a new green approach for biosynthesis of silver nanoparticles, myconanotechnology has been represented as a novel field of study in nanotechnology. In this study, we have reported the extracellular synthesis of highly stable silver nanoparticles using three species of dermatophytes: Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis. METHODS: Clinical strains of these species were grown in a liquid medium containing mineral salt and incubated at 25°C for 5-7 days. The cell-free filtrate of each culture was obtained and subjected to synthesize silver nanoparticles in the presence of 1 mM AgNO3. RESULTS: The reduction of Ag+ ions in metal nanoparticles was investigated virtually by tracing the solution color which was switched into reddish-light brown after 72 h. For T. mentagrophytes, a UV-visible spectra demonstrating a strong, quite narrow peak located between 422 and 425 nm was obtained. For M. canis, a fairly wide peak centering at 441 nm and for T. rubrum, a weak spectrum to decipher were observed. According to transmission electron microscopy (TEM) results, fairly uniform, spherical, and small in size with almost less than 50 nm particles were forms in case of T. mentagrophytes. For the other two species, TEM images showed existence of small spherical nanosilvers but not as small as nanoparticles synthesized by T. mentagrophytes. CONCLUSION: We observed that species belong to a single genus of the fungi have variable ability to synthesize silver nanoparticles extracellulary with different efficiency. Furthermore, the extracellular synthesis may make the process simpler and easier for following processes.
BACKGROUND: To develop a new green approach for biosynthesis of silver nanoparticles, myconanotechnology has been represented as a novel field of study in nanotechnology. In this study, we have reported the extracellular synthesis of highly stable silver nanoparticles using three species of dermatophytes: Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis. METHODS: Clinical strains of these species were grown in a liquid medium containing mineral salt and incubated at 25°C for 5-7 days. The cell-free filtrate of each culture was obtained and subjected to synthesize silver nanoparticles in the presence of 1 mM AgNO3. RESULTS: The reduction of Ag+ ions in metal nanoparticles was investigated virtually by tracing the solution color which was switched into reddish-light brown after 72 h. For T. mentagrophytes, a UV-visible spectra demonstrating a strong, quite narrow peak located between 422 and 425 nm was obtained. For M. canis, a fairly wide peak centering at 441 nm and for T. rubrum, a weak spectrum to decipher were observed. According to transmission electron microscopy (TEM) results, fairly uniform, spherical, and small in size with almost less than 50 nm particles were forms in case of T. mentagrophytes. For the other two species, TEM images showed existence of small spherical nanosilvers but not as small as nanoparticles synthesized by T. mentagrophytes. CONCLUSION: We observed that species belong to a single genus of the fungi have variable ability to synthesize silver nanoparticles extracellulary with different efficiency. Furthermore, the extracellular synthesis may make the process simpler and easier for following processes.
Authors: S Anil Kumar; Majid Kazemian Abyaneh; S W Gosavi; Sulabha K Kulkarni; Renu Pasricha; Absar Ahmad; M I Khan Journal: Biotechnol Lett Date: 2007-01-20 Impact factor: 2.461
Authors: H-J Schmoll; C Kollmannsberger; B Metzner; J T Hartmann; N Schleucher; P Schöffski; J Schleicher; O Rick; J Beyer; D Hossfeld; L Kanz; W E Berdel; R Andreesen; C Bokemeyer Journal: J Clin Oncol Date: 2003-10-20 Impact factor: 44.544
Authors: Laryssa Pinheiro Costa Silva; Jairo Pinto Oliveira; Wanderson Juvencio Keijok; André Romero da Silva; Anderson Rocha Aguiar; Marco Cesar Cunegundes Guimarães; Carolina Magri Ferraz; Jackson Victor Araújo; Fernando Luiz Tobias; Fábio Ribeiro Braga Journal: Int J Nanomedicine Date: 2017-08-31
Authors: Anna Claudia Mombrini Silva Barbosa; Laryssa Pinheiro Costa Silva; Carolina Magri Ferraz; Fernando Luiz Tobias; Jackson Victor de Araújo; Barbara Loureiro; Gracilene Maria Almeida Muniz Braga; Francielle Bosi Rodrigues Veloso; Filippe Elias de Freitas Soares; Marcio Fronza; Fabio Ribeiro Braga Journal: Int J Nanomedicine Date: 2019-04-02