| Literature DB >> 22561985 |
Keum-Soo Song1, Satish Balasaheb Nimse, Heejung An, Junghoon Kim, Van-Thuan Nguyen, Van-Thao Ta, Taisun Kim.
Abstract
The novel HPV 9G DNAChips were developed for the detection and discrimination of the HPV genotypes in the clinical samples. The HPV 9G DNAChip established high SBR of 50-70 and 100% target-specific hybridization after 30min hybridization and 2×2min washing at 25°C. We compared the genotyping results of the 959 HPV positive and 82 HPV negative clinical samples by the HPV 9G DNAChip and the sequencing; the results are in 100% agreement. The HPV 9G DNAChip efficiently discriminate 19 HPV genotypes in the 959 HPV positive clinical samples. The results of HPV 9G DNAChip were 100% identical with the sequencing analysis in the detection and discrimination of HPV genotypes in the HPV negative clinical samples. The high SBR, 100% target-specific hybridization, and 25°C hybridization and washing makes the HPV 9G DNAChip a promising diagnostic tool for the accurate HPV genotyping.Entities:
Mesh：
Substances：
Year: 2012 PMID： 22561985 DOI： 10.1016/j.jviromet.2012.04.003
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014