Literature DB >> 22561095

High-resolution melting analysis for rapid detection of linezolid resistance (mediated by G2576T mutation) in Staphylococcus epidermidis.

Emma M Gabriel1, Pierre E Douarre, Siobhan Fitzgibbon, James Clair, Brigid Lucey, Aidan Coffey, Jim M O'Mahony.   

Abstract

We describe a novel HRM-PCR (high-resolution melting) assay capable of the accurate identification of the G2576T point mutation in domain V of the 23S rRNA genes attributed to linezolid resistance in Staphylococcus epidermidis. This rapid method demonstrated 100% correlation with the previously established NheI restriction digest assay.
Copyright © 2012 Elsevier B.V. All rights reserved.

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Year:  2012        PMID: 22561095     DOI: 10.1016/j.mimet.2012.04.002

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN: 0167-7012            Impact factor:   2.363


  2 in total

1.  A commentary on the role of molecular technology and automation in clinical diagnostics.

Authors:  Ciara O'Connor; Marie Fitzgibbon; James Powell; Denis Barron; Jim O'Mahony; Lorraine Power; Nuala H O'Connell; Colum Dunne
Journal:  Bioengineered       Date:  2014-03-21       Impact factor: 3.269

2.  Linezolid-resistant Enterococcus faecium strains isolated from one hospital in Poland -commensals or hospital-adapted pathogens?

Authors:  Beata Krawczyk; Magdalena Wysocka; Roman Kotłowski; Marek Bronk; Michał Michalik; Alfred Samet
Journal:  PLoS One       Date:  2020-05-26       Impact factor: 3.240

  2 in total

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