| Literature DB >> 22554577 |
P Pereiro1, A Martinez-Lopez, A Falco, S Dios, A Figueras, J M Coll, B Novoa, A Estepa.
Abstract
Turbot (Scophthalmus maximus) is a high-value farmed marine flatfish with growing demand and production levels in Europe susceptible to turbot-specific viral haemorrhagic septicaemia virus (VHSV) strains. To evaluate the possibility of controlling the outbreaks of this infectious disease by means of DNA vaccination, the gpG of a VHSV isolated from farmed turbot (VHSV(860)) was cloned into an expression plasmid containing the human cytomegalovirus (CMV) promoter (pMCV1.4-G(860)). In our experimental conditions, DNA immunised turbots were more than 85% protected against VHSV(860) lethal challenge and showed both VHSV-gpG specific and neutralizing antibodies. To our knowledge this is the first report showing the efficacy of turbot genetic immunisation against a VHSV. Work is in progress to determine the contribution of innate and adaptive immunity to the protective response elicited by the immunization.Entities:
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Year: 2012 PMID： 22554577 DOI： 10.1016/j.fsi.2012.03.004
Source DB: PubMed Journal: Fish Shellfish Immunol ISSN： 1050-4648 Impact factor: 4.581