Yun Wang1, Juan Tan, Ye-Hong Zhuo, Cui-Lan Wang, Xu-Yang Liu, Su-Ping Cai. 1. Ophthalmic Laboratories & Department of Ophthalmology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China.
Abstract
AIM: To determine the effects of a low dose latrunculin (LAT)-A on dexamethasone (Dex)-induced upregulation of extracellular matrix proteins fibronectin (FN) in cultured human trabecular meshwork (HTM) cells. METHODS: HTM cells were cultured to confluent and incubated with 0.4µmol/L Dex and/or 0.05µmol/L LAT-A. FN expression in HTM cells was evaluated by Western blot and immunofluorescence microscopy. RESULTS: Dex up-regulated FN production in HTM cells, failed to do so when co-incubated with LAT-A. LAT-A decreased production of FN in cultured HTM cells. CONCLUSION: This study indicated that LAT-A may modulate the expression of fibronectin in trabecular meshwork to achieve treatment for steroids and other types of glaucoma. It has an important prospect as an intraocular pressure- lowering drug.
AIM: To determine the effects of a low dose latrunculin (LAT)-A on dexamethasone (Dex)-induced upregulation of extracellular matrix proteins fibronectin (FN) in cultured human trabecular meshwork (HTM) cells. METHODS: HTM cells were cultured to confluent and incubated with 0.4µmol/L Dex and/or 0.05µmol/L LAT-A. FN expression in HTM cells was evaluated by Western blot and immunofluorescence microscopy. RESULTS:Dex up-regulated FN production in HTM cells, failed to do so when co-incubated with LAT-A. LAT-A decreased production of FN in cultured HTM cells. CONCLUSION: This study indicated that LAT-A may modulate the expression of fibronectin in trabecular meshwork to achieve treatment for steroids and other types of glaucoma. It has an important prospect as an intraocular pressure- lowering drug.
Entities:
Keywords:
dexamethasone; fibronectin; human trabecular meshwork cells; latrunculin-A
Authors: Mark S Filla; Marie K Schwinn; Amanda K Nosie; Ross W Clark; Donna M Peters Journal: Invest Ophthalmol Vis Sci Date: 2011-05-05 Impact factor: 4.799
Authors: Mary-Jo Hoare; Ian Grierson; Daniel Brotchie; Natalie Pollock; Kathy Cracknell; Abbot F Clark Journal: Invest Ophthalmol Vis Sci Date: 2008-10-24 Impact factor: 4.799