Chao Wan1, Ning-Ning Liu, Li-Min Liu, Na Cai, Lei Chen. 1. Department of Ophthalmology, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
Abstract
AIM: To observe effect of adenovirus-mediated brain derived neurotrophic factor in early retinal neuropathy of diabetes in rats. METHODS: Adult male Wistar rats, 9 weeks of age, were injected intraperitoneally with STZ to induce diabetes. Two weeks after the models were established, Ad.BDNF was administered into the vitreous cavities of rats. Four weeks after the models were set up, the rats were killed and the retina was removed for Western blotting and whole-mount immunohistochemistry for tyrosine hydroxylase (TH) to observe the changes of TH and dopaminergic amacrine cells in retina. RESULTS: The protein levels of TH and the number of positive staining dopaminergic amacrine cells and the staining gray scale of experimental group without Ad.BDNF were lower statistically. But there was no statistically significant difference between the experimental group with Ad.BDNF and control group. CONCLUSION: In the early stage of STZ diabetic, the administration of Ad.BDNF into the vitreous cavities can increase TH protein levels and the density of dopaminergic amacrine cells in the STZ rats' retina.
AIM: To observe effect of adenovirus-mediated brain derived neurotrophic factor in early retinal neuropathy of diabetes in rats. METHODS: Adult male Wistar rats, 9 weeks of age, were injected intraperitoneally with STZ to induce diabetes. Two weeks after the models were established, Ad.BDNF was administered into the vitreous cavities of rats. Four weeks after the models were set up, the rats were killed and the retina was removed for Western blotting and whole-mount immunohistochemistry for tyrosine hydroxylase (TH) to observe the changes of TH and dopaminergic amacrine cells in retina. RESULTS: The protein levels of TH and the number of positive staining dopaminergic amacrine cells and the staining gray scale of experimental group without Ad.BDNF were lower statistically. But there was no statistically significant difference between the experimental group with Ad.BDNF and control group. CONCLUSION: In the early stage of STZdiabetic, the administration of Ad.BDNF into the vitreous cavities can increase TH protein levels and the density of dopaminergic amacrine cells in the STZrats' retina.
Authors: M Nakamura; A J Barber; D A Antonetti; K F LaNoue; K A Robinson; M G Buse; T W Gardner Journal: J Biol Chem Date: 2001-09-17 Impact factor: 5.157
Authors: Marta Agudo; Maria Cruz Pérez-Marín; Ulrika Lönngren; Paloma Sobrado; Ana Conesa; Isabel Cánovas; Manuel Salinas-Navarro; Jaime Miralles-Imperial; Finn Hallböök; Manuel Vidal-Sanz Journal: Mol Vis Date: 2008-06-03 Impact factor: 2.367
Authors: Ahmed M Abu El-Asrar; Mohammad Mairaj Siddiquei; Mohd Imtiaz Nawaz; Karel Geboes; Ghulam Mohammad Journal: Mediators Inflamm Date: 2014-03-10 Impact factor: 4.711