Ling-Ling Yang1, Qing-Jun Zhou, Yao Wang, Yi-Qiang Wang. 1. State Key Laboratory Cultivation Base, Shandong Provincial Key Lab of Ophthalmology, Shandong Eye Institute, Qingdao 266071, Shandong Province, China.
Abstract
AIM: To detect the differentiation effects of retinal cells or extracts on bone marrow-derived mesenchymal stem cells (BMSC). METHODS: Human fetal BMSC were previously labelled by carboxyfluorescein succinimidyl ester (CFSE), and co-cultured with retinal pigment epithelial (RPE) cells which were pre-treated with ultraviolet irradiation at a ratio of 1:1 to induce the differentiation of BMSC for up to 14 days. In some assays, a retinal extract of bovine retinal extract (BRE) was added to detect the potential effects of retinal component on the differentiation of BMSC. In addition, Neuron-specific enolase (NSE), Nestin and Glial fibrillary acidic protein (GFAP) immunostaining were performed to determine the characteristics of BMSC. RESULTS: The results indicated that by co-cultured with RPE cells, fetal BMSC were differentiated into neural-like cells expressing special neuronal markers Nestin, GFAP and NSE. And the expression of these markers was obviously increased by BRE. CONCLUSION: Retina derived cells and extracts can induce the differentiation of BMSC into neural-like cells.
AIM: To detect the differentiation effects of retinal cells or extracts on bone marrow-derived mesenchymal stem cells (BMSC). METHODS:Human fetal BMSC were previously labelled by carboxyfluorescein succinimidyl ester (CFSE), and co-cultured with retinal pigment epithelial (RPE) cells which were pre-treated with ultraviolet irradiation at a ratio of 1:1 to induce the differentiation of BMSC for up to 14 days. In some assays, a retinal extract of bovine retinal extract (BRE) was added to detect the potential effects of retinal component on the differentiation of BMSC. In addition, Neuron-specific enolase (NSE), Nestin and Glial fibrillary acidic protein (GFAP) immunostaining were performed to determine the characteristics of BMSC. RESULTS: The results indicated that by co-cultured with RPE cells, fetal BMSC were differentiated into neural-like cells expressing special neuronal markers Nestin, GFAP and NSE. And the expression of these markers was obviously increased by BRE. CONCLUSION: Retina derived cells and extracts can induce the differentiation of BMSC into neural-like cells.
Authors: Pooi Ling Mok; Sue Ngein Leow; Avin Ee-Hwan Koh; Hairul Harun Mohd Nizam; Suet Lee Shirley Ding; Chi Luu; Raduan Ruhaslizan; Hon Seng Wong; Wan Haslina Wan Abdul Halim; Min Hwei Ng; Ruszymah Binti Hj Idrus; Shiplu Roy Chowdhury; Catherine Mae-Lynn Bastion; Suresh Kumar Subbiah; Akon Higuchi; Abdullah A Alarfaj; Kong Yong Then Journal: Int J Mol Sci Date: 2017-02-08 Impact factor: 5.923