OBJECTIVE: To immunize rabbits with 12 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus (E. granulosus) and measuring polyclonal antibody and humoral immune response using ELISA and gel diffusion. METHODS: Two mentioned antigens were cloned and expressed in expression vector and purified by affinity chromatography. Four young rabbits were selected and challenged intradermally with yielded recombinant antigens. Rabbits' sera were collected post infection and were tested using ELISA and gel diffusion for polyclonal antibody detection 10 days after last injection. RESULTS: The specific antibody against the recombinant peptides was efficiently produced within 4 weeks post infection. CONCLUSIONS: Produced recombinants proteins could induce the immune response of the rabbits successfully. This process might improve the clarification of diagnosis and vaccination as regards hydatidosis.
OBJECTIVE: To immunize rabbits with 12 and 16 kDa recombinant subunits of antigen B from Echinococcus granulosus (E. granulosus) and measuring polyclonal antibody and humoral immune response using ELISA and gel diffusion. METHODS: Two mentioned antigens were cloned and expressed in expression vector and purified by affinity chromatography. Four young rabbits were selected and challenged intradermally with yielded recombinant antigens. Rabbits' sera were collected post infection and were tested using ELISA and gel diffusion for polyclonal antibody detection 10 days after last injection. RESULTS: The specific antibody against the recombinant peptides was efficiently produced within 4 weeks post infection. CONCLUSIONS: Produced recombinants proteins could induce the immune response of the rabbits successfully. This process might improve the clarification of diagnosis and vaccination as regards hydatidosis.
Authors: Hakim Azizi; Bahram Kazemi; Mojgan Bandehpour; Mehdi Mohebali; Ali Khamesipour; Mojgan Aryaeipour; Mohammad Bagher Rokni Journal: Iran J Public Health Date: 2015-07 Impact factor: 1.429