Literature DB >> 22544240

A 50-kilodalton Cry2A peptide is lethal to Bombyx mori and Lymantria dispar.

Masataka Ohsawa1, Miki Tanaka, Kenta Moriyama, Mitsuaki Shimazu, Shin-ichiro Asano, Kazuhisa Miyamoto, Kohsuke Haginoya, Toshiaki Mitsui, Tomoaki Kouya, Masayuki Taniguchi, Hidetaka Hori.   

Abstract

The Cry2Aa3 gene was introduced into asporogenic Bacillus thuringiensis, and the synthesized protoxin killed Bombyx mori and Lymantria dispar larvae. Chymotrypsin hydrolyzed the linkages between 49Tyr/Val50 and 145Lys/Ser146 in the protoxin, and 50- and 58-kDa fragments were generated, respectively. Both peptides killed the larvae of both insects.

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Year:  2012        PMID： 22544240      PMCID： PMC3370495          DOI： 10.1128/AEM.07123-11

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  14 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  Characterization of a cry2A gene cloned from an isolate of Bacillus thuringiensis serovar sotto.

Authors:  J Sasaki; S Asano; N Hashimoto; B W Lay; S Hastowo; H Bando; T Iizuka
Journal:  Curr Microbiol       Date:  1997-07       Impact factor: 2.188

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

Review 4.  Biochemistry and genetics of insect resistance to Bacillus thuringiensis.

Authors:  Juan Ferré; Jeroen Van Rie
Journal:  Annu Rev Entomol       Date:  2002       Impact factor: 19.686

5.  Bacillus thuringiensis CryIA(a) insecticidal toxin: crystal structure and channel formation.

Authors:  P Grochulski; L Masson; S Borisova; M Pusztai-Carey; J L Schwartz; R Brousseau; M Cygler
Journal:  J Mol Biol       Date:  1995-12-01       Impact factor: 5.469

6.  Isolation of a protein from the parasporal crystal of Bacillus thuringiensis var. Kurstaki toxic to the mosquito larva, Aedes taeniorhynchus.

Authors:  T Yamamoto; R E McLaughlin
Journal:  Biochem Biophys Res Commun       Date:  1981-11-30       Impact factor: 3.575

7.  Structure of Cry2Aa suggests an unexpected receptor binding epitope.

Authors:  R J Morse; T Yamamoto; R M Stroud
Journal:  Structure       Date:  2001-05-09       Impact factor: 5.006

Review 8.  Bacillus thuringiensis and its pesticidal crystal proteins.

Authors:  E Schnepf; N Crickmore; J Van Rie; D Lereclus; J Baum; J Feitelson; D R Zeigler; D H Dean
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

9.  Control of resistant pink bollworm (Pectinophora gossypiella) by transgenic cotton that produces Bacillus thuringiensis toxin Cry2Ab.

Authors:  Bruce E Tabashnik; Timothy J Dennehy; Maria A Sims; Karen Larkin; Graham P Head; William J Moar; Yves Carrière
Journal:  Appl Environ Microbiol       Date:  2002-08       Impact factor: 4.792

10.  Specific binding of Bacillus thuringiensis Cry2A insecticidal proteins to a common site in the midgut of Helicoverpa species.

Authors:  Carmen Sara Hernández-Rodríguez; Adri Van Vliet; Nadine Bautsoens; Jeroen Van Rie; Juan Ferré
Journal:  Appl Environ Microbiol       Date:  2008-10-17       Impact factor: 4.792
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  2 in total

1.  Proteolysis activation of Cry1Ac and Cry2Ab protoxins by larval midgut juice proteases from Helicoverpa armigera.

Authors:  Shaoyan Liu; Shuo Wang; Shuwen Wu; Yidong Wu; Yihua Yang
Journal:  PLoS One       Date:  2020-01-31       Impact factor: 3.240

2.  Insect Resistance to Bacillus thuringiensis Toxin Cry2Ab Is Conferred by Mutations in an ABC Transporter Subfamily A Protein.

Authors:  Wee Tek Tay; Rod J Mahon; David G Heckel; Thomas K Walsh; Sharon Downes; William J James; Sui-Fai Lee; Annette Reineke; Adam K Williams; Karl H J Gordon
Journal:  PLoS Genet       Date:  2015-11-19       Impact factor: 5.917
  2 in total

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