Literature DB >> 225439

A simple immunofluorescent technique for the detection of human rotavirus.

R B Moosai, P S Gardner, J D Almeida, M A Greenaway.   

Abstract

If trypsin is incorporated in the tissue culture medium it is possible to carry out a sensitive immunofluorescence assay for the presence of human rotavirus. The enhanced effect of trypsin is negated by serum. It has also been established that naturally occurring enzymes in faeces enable some virus to penetrate tissue culture cells. The role of these naturally occurring enzymes in the pathogenesis of rotavirus infection is discussed.

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Year:  1979        PMID： 225439     DOI： 10.1002/jmv.1890030304

Source DB:  PubMed          Journal:  J Med Virol        ISSN： 0146-6615            Impact factor:   2.327


  9 in total

1.  Detection of rotavirus in sewage.

Authors:  J Steinmann
Journal:  Appl Environ Microbiol       Date:  1981-04       Impact factor: 4.792

2.  Detection of rotavirus by a latex agglutination test, Rotalex: comparison with electron microscopy, immunofluorescence, polyacrylamide gel electrophoresis, and enzyme linked immunosorbent assay.

Authors:  R B Moosai; R Alcock; T M Bell; F R Laidler; J S Peiris; A P Wyn-Jones; C R Madeley
Journal:  J Clin Pathol       Date:  1985-06       Impact factor: 3.411

3.  Trypsin enhancement of rotavirus infectivity: mechanism of enhancement.

Authors:  S M Clark; J R Roth; M L Clark; B B Barnett; R S Spendlove
Journal:  J Virol       Date:  1981-09       Impact factor: 5.103

4.  Rapid detection of enteric adenovirus and rotavirus: a simple method using polyacrylamide gel electrophoresis.

Authors:  R B Moosai; M J Carter; C R Madeley
Journal:  J Clin Pathol       Date:  1984-12       Impact factor: 3.411

5.  Structural polypeptides of simian rotavirus SA11 and the effect of trypsin.

Authors:  R T Espejo; S López; C Arias
Journal:  J Virol       Date:  1981-01       Impact factor: 5.103

6.  Comparison of methods for rotavirus detection in water and results of a survey of Jerusalem wastewater.

Authors:  N Guttman-Bass; Y Tchorsh; E Marva
Journal:  Appl Environ Microbiol       Date:  1987-04       Impact factor: 4.792

7.  Proteolytic enzymes and rotavirus SA-11 plaque formation.

Authors:  S Ramia; S A Sattar
Journal:  Can J Comp Med       Date:  1980-04

8.  Effects of antibodies, trypsin, and trypsin inhibitors on susceptibility of neonates to rotavirus infection.

Authors:  B S McLean; I H Holmes
Journal:  J Clin Microbiol       Date:  1981-01       Impact factor: 5.948

Review 9.  Viral gastroenteritis.

Authors:  B Barnett
Journal:  Med Clin North Am       Date:  1983-09       Impact factor: 5.456
  9 in total

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