ETHNOPHARMACOLOGICAL RELEVANCE: Labisia pumila var. alata (LP) is a phytoestrogenic herb with potential as an alternative to Estrogen Replacement Therapy (ERT) in the treatment of postmenopausal osteoporosis. LP has been reported to produce similar effects to ERT on the bone markers, but could not match ERT in terms of maintaining the bone calcium in postmenopausal osteoporosis rat model. This study aimed to examine in detail the effects of LP on the bone of postmenopausal osteoporosis rat model using bone histomorphometry. MATERIALS AND METHODS: Thirty two female rats were randomly divided into groups of: Sham operated (Sham), ovariectomized control (OVXC), ovariectomized with Labisia pumila var. alata (LP) and ovariectomized with ERT (Premarin®) (ERT). The LP and ERT were administered through the route of oral gavage daily at the dose of 17.5 mg/kg and 64.5 μg/kg respectively. Following 2 months of treatment, rats were euthanized and the left femurs were dissected out and prepared for bone histomorphometry. RESULTS: Histomorphometric analysis revealed osteoporotic changes for the ovariectomized rats. Supplementation of LP to ovariectomized rats could prevent these osteoporotic changes, as effective as ERT. CONCLUSION: This confirmed that LP has potential as an alternative to ERT for prevention of postmenopausal osteoporosis.
ETHNOPHARMACOLOGICAL RELEVANCE: Labisia pumila var. alata (LP) is a phytoestrogenic herb with potential as an alternative to Estrogen Replacement Therapy (ERT) in the treatment of postmenopausal osteoporosis. LP has been reported to produce similar effects to ERT on the bone markers, but could not match ERT in terms of maintaining the bone calcium in postmenopausal osteoporosisrat model. This study aimed to examine in detail the effects of LP on the bone of postmenopausal osteoporosisrat model using bone histomorphometry. MATERIALS AND METHODS: Thirty two female rats were randomly divided into groups of: Sham operated (Sham), ovariectomized control (OVXC), ovariectomized with Labisia pumila var. alata (LP) and ovariectomized with ERT (Premarin®) (ERT). The LP and ERT were administered through the route of oral gavage daily at the dose of 17.5 mg/kg and 64.5 μg/kg respectively. Following 2 months of treatment, rats were euthanized and the left femurs were dissected out and prepared for bone histomorphometry. RESULTS: Histomorphometric analysis revealed osteoporotic changes for the ovariectomized rats. Supplementation of LP to ovariectomized rats could prevent these osteoporotic changes, as effective as ERT. CONCLUSION: This confirmed that LP has potential as an alternative to ERT for prevention of postmenopausal osteoporosis.
Authors: Iffah Nadhira Madzuki; Seng Fong Lau; Nur Adeelah Che Ahmad Tantowi; Nur Iliyani Mohd Ishak; Suhaila Mohamed Journal: Inflammopharmacology Date: 2018-02-19 Impact factor: 4.473
Authors: Nadia Mohd Effendy; Shahrum Abdullah; Mohd Faridz Mod Yunoh; Ahmad Nazrun Shuid Journal: BMC Complement Altern Med Date: 2015-03-12 Impact factor: 3.659
Authors: James Jam Jolly; Kok-Yong Chin; Ekram Alias; Kien Hui Chua; Ima Nirwana Soelaiman Journal: Int J Environ Res Public Health Date: 2018-05-11 Impact factor: 3.390