Literature DB >> 22540128

Stepping towards highly flexible aptamers: enzymatic recognition studies of unlocked nucleic acid nucleotides.

Camille Dubois1, Meghan A Campbell, Stacey L Edwards, Jesper Wengel, Rakesh N Veedu.   

Abstract

Enzymatic recognition of unlocked nucleic acid (UNA) nucleotides was successfully accomplished. Therminator DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of a 81 mer UNA-modified DNA library was efficiently achieved by KOD DNA polymerase.

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Year:  2012        PMID： 22540128     DOI： 10.1039/c2cc31316b

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  3 in total

1.  Unlocked nucleic acid modified primer-based enzymatic polymerization assay: towards allele-specific genotype detection of human platelet antigens.

Authors:  Bao T Le; Quintin Hughes; Shilpa Rakesh; Ross Baker; Per T Jørgensen; Jesper Wengel; Rakesh N Veedu
Journal:  RSC Adv       Date:  2018-09-21       Impact factor: 4.036

Review 2.  In vitro selection of BNA (LNA) aptamers.

Authors:  Masayasu Kuwahara; Satoshi Obika
Journal:  Artif DNA PNA XNA       Date:  2013 Apr-Jun

Review 3.  Therminator DNA Polymerase: Modified Nucleotides and Unnatural Substrates.

Authors:  Andrew F Gardner; Kiserian M Jackson; Madeleine M Boyle; Jackson A Buss; Vladimir Potapov; Alexandra M Gehring; Kelly M Zatopek; Ivan R Corrêa; Jennifer L Ong; William E Jack
Journal:  Front Mol Biosci       Date:  2019-04-24
  3 in total

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