Zuozhou Li1, Qingxiang Han, Yuanyuan Chen, Wei Li. 1. Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei, People's Republic of China. lizz@wbgcas.cn
Abstract
PREMISE OF THE STUDY: The first microsatellite primers were developed for Isoetes hypsophila, an endangered quillwort species endemic to the Qinghai-Tibetan Plateau in China, to further describe its genetic variability and population structure. We also examined their cross-amplification in a congeneric species, I. sinensis. METHODS AND RESULTS: Using the Fast Isolation by AFLP of Sequences COntaining Repeats (FIASCO) protocol, nine microsatellite loci were isolated and characterized in 32 samples from four natural populations of I. hypsophila. The primers amplified di- and hexanucleotide repeats with three to 11 alleles per locus. Seven of nine primers were cross-amplified in I. sinensis with two to seven alleles per locus. CONCLUSION: The microsatellite loci primers will be useful for studies of genetic diversity and gene flow in natural populations of Isoetes species.
PREMISE OF THE STUDY: The first microsatellite primers were developed for Isoetes hypsophila, an endangered quillwort species endemic to the Qinghai-Tibetan Plateau in China, to further describe its genetic variability and population structure. We also examined their cross-amplification in a congeneric species, I. sinensis. METHODS AND RESULTS: Using the Fast Isolation by AFLP of Sequences COntaining Repeats (FIASCO) protocol, nine microsatellite loci were isolated and characterized in 32 samples from four natural populations of I. hypsophila. The primers amplified di- and hexanucleotide repeats with three to 11 alleles per locus. Seven of nine primers were cross-amplified in I. sinensis with two to seven alleles per locus. CONCLUSION: The microsatellite loci primers will be useful for studies of genetic diversity and gene flow in natural populations of Isoetes species.
Authors: Mirella Pupo Santos; João V S Rabelo Araujo; Arthur V Sant'anna Lopes; Julio Cesar Fiorio Vettorazzi; Marcela Santana Bastos Boechat; Fernanda AbreuSantana Arêdes; Naiara Viana Campos; Emiliano Nicolas Calderon; Fernando M Gomes Santos; Tais Nogueira Fernandes; Rodrigo Nunes da Fonseca; Messias Gonzaga Pereira; Guilherme Oliveira; Daniel Basilio Zandonadi; RodrigoLemes Martins; Francisco de Assis Esteves Journal: PeerJ Date: 2020-11-09 Impact factor: 2.984