BACKGROUND: Prostate acinar epithelial cells release microvesicles (prostasomes) that possess pleiotropic biological effects relevant for successful fertilization. Prostasomes are formed in a similar way as exosomes but are heterogeneous as regards size and appearance. Like exosomes they are thought to be mediators of intercellular communication. METHODS: We prepared seminal prostasomes in accordance with the prevailing protocol for exosome preparation including passage through a 0.2 µm filter and centrifugation in a sucrose gradient. RESULTS: We compared the "filterable prostasomes" with those trapped on the filter ("nonfilterable prostasomes") and, qualitatively, no conspicuous differences were apparent regarding ultrastructure and SDS-PAGE banding pattern. Moreover, both types of prostasomes contained DNA fragments and Western blot revealed presence of prostate specific membrane antigen (PSMA), CD38, and annexin A1. CONCLUSIONS: Reasonably, prostasomes could be included in the exosome family and be regarded as one entity containing chromosomal DNA.
BACKGROUND: Prostate acinar epithelial cells release microvesicles (prostasomes) that possess pleiotropic biological effects relevant for successful fertilization. Prostasomes are formed in a similar way as exosomes but are heterogeneous as regards size and appearance. Like exosomes they are thought to be mediators of intercellular communication. METHODS: We prepared seminal prostasomes in accordance with the prevailing protocol for exosome preparation including passage through a 0.2 µm filter and centrifugation in a sucrose gradient. RESULTS: We compared the "filterable prostasomes" with those trapped on the filter ("nonfilterable prostasomes") and, qualitatively, no conspicuous differences were apparent regarding ultrastructure and SDS-PAGE banding pattern. Moreover, both types of prostasomes contained DNA fragments and Western blot revealed presence of prostate specific membrane antigen (PSMA), CD38, and annexin A1. CONCLUSIONS: Reasonably, prostasomes could be included in the exosome family and be regarded as one entity containing chromosomal DNA.
Authors: Subhash Chand; Austin Gowen; Mason Savine; Dalia Moore; Alexander Clark; Wendy Huynh; Niming Wu; Katherine Odegaard; Lucas Weyrich; Rick A Bevins; Howard S Fox; Gurudutt Pendyala; Sowmya V Yelamanchili Journal: J Extracell Vesicles Date: 2021-12
Authors: Julius O Nyalwidhe; Lucy R Betesh; Thomas W Powers; E Ellen Jones; Krista Y White; Tanya C Burch; Jasmin Brooks; Megan T Watson; Raymond S Lance; Dean A Troyer; O John Semmes; Anand Mehta; Richard R Drake Journal: Proteomics Clin Appl Date: 2013-09-13 Impact factor: 3.494
Authors: Ekaterina A Alyamkina; Valeriy P Nikolin; Nelly A Popova; Alexandra M Minkevich; Artem V Kozel; Evgenia V Dolgova; Yaroslav R Efremov; Sergey I Bayborodin; Oleg M Andrushkevich; Oleg S Taranov; Vladimir V Omigov; Vladimir A Rogachev; Anastasia S Proskurina; Evgeniy I Vereschagin; Elena V Kiseleva; Maria V Zhukova; Alexandr A Ostanin; Elena R Chernykh; Sergey S Bogachev; Mikhail A Shurdov Journal: Cancer Cell Int Date: 2015-03-19 Impact factor: 5.722