Literature DB >> 22535774

Use of influenza A viruses expressing reporter genes to assess the frequency of double infections in vitro.

R Bodewes1, N J Nieuwkoop1, R J Verburgh1, R A M Fouchier1, A D M E Osterhaus2,1, G F Rimmelzwaan2,1.   

Abstract

Exchange of gene segments between mammalian and avian influenza A viruses may lead to the emergence of potential pandemic influenza viruses. Since co-infection of single cells with two viruses is a prerequisite for reassortment to take place, we assessed frequencies of double-infection in vitro using influenza A/H5N1 and A/H1N1 viruses expressing the reporter genes eGFP or mCherry. Double-infected A549 and Madin-Darby canine kidney cells were detected by confocal microscopy and flow cytometry.

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Year:  2012        PMID: 22535774     DOI: 10.1099/vir.0.042671-0

Source DB:  PubMed          Journal:  J Gen Virol        ISSN: 0022-1317            Impact factor:   3.891


  9 in total

1.  Competitive detection of influenza neutralizing antibodies using a novel bivalent fluorescence-based microneutralization assay (BiFMA).

Authors:  Steven F Baker; Aitor Nogales; Felix W Santiago; David J Topham; Luis Martínez-Sobrido
Journal:  Vaccine       Date:  2015-06-01       Impact factor: 3.641

2.  Human cytotoxic T lymphocytes directed to seasonal influenza A viruses cross-react with the newly emerging H7N9 virus.

Authors:  Carolien E van de Sandt; Joost H C M Kreijtz; Gerrie de Mutsert; Martina M Geelhoed-Mieras; Marine L B Hillaire; Stella E Vogelzang-van Trierum; Albert D M E Osterhaus; Ron A M Fouchier; Guus F Rimmelzwaan
Journal:  J Virol       Date:  2013-11-20       Impact factor: 5.103

Review 3.  Development and applications of single-cycle infectious influenza A virus (sciIAV).

Authors:  Aitor Nogales; Steven F Baker; William Domm; Luis Martínez-Sobrido
Journal:  Virus Res       Date:  2015-07-26       Impact factor: 3.303

4.  Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes.

Authors:  Aitor Nogales; Michael Schotsaert; Raveen Rathnasinghe; Marta L DeDiego; Adolfo García-Sastre; Luis Martinez-Sobrido
Journal:  Viruses       Date:  2021-04-17       Impact factor: 5.048

5.  Reassortment between Avian H5N1 and human influenza viruses is mainly restricted to the matrix and neuraminidase gene segments.

Authors:  Eefje J A Schrauwen; Theo M Bestebroer; Guus F Rimmelzwaan; Albert D M E Osterhaus; Ron A M Fouchier; Sander Herfst
Journal:  PLoS One       Date:  2013-03-20       Impact factor: 3.240

6.  Influenza virus reassortment occurs with high frequency in the absence of segment mismatch.

Authors:  Nicolle Marshall; Lalita Priyamvada; Zachary Ende; John Steel; Anice C Lowen
Journal:  PLoS Pathog       Date:  2013-06-13       Impact factor: 6.823

Review 7.  Evasion of influenza A viruses from innate and adaptive immune responses.

Authors:  Carolien E van de Sandt; Joost H C M Kreijtz; Guus F Rimmelzwaan
Journal:  Viruses       Date:  2012-09-03       Impact factor: 5.048

8.  Optimisations and Challenges Involved in the Creation of Various Bioluminescent and Fluorescent Influenza A Virus Strains for In Vitro and In Vivo Applications.

Authors:  Monique I Spronken; Kirsty R Short; Sander Herfst; Theo M Bestebroer; Vincent P Vaes; Barbara van der Hoeven; Abraham J Koster; Gert-Jan Kremers; Dana P Scott; Alexander P Gultyaev; Erin M Sorell; Miranda de Graaf; Montserrat Bárcena; Guus F Rimmelzwaan; Ron A Fouchier
Journal:  PLoS One       Date:  2015-08-04       Impact factor: 3.240

Review 9.  RNA Virus Reassortment: An Evolutionary Mechanism for Host Jumps and Immune Evasion.

Authors:  Dhanasekaran Vijaykrishna; Reshmi Mukerji; Gavin J D Smith
Journal:  PLoS Pathog       Date:  2015-07-09       Impact factor: 6.823

  9 in total

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