OBJECTIVES: Our objective was to develop an experimental model for the noninvasive and objective evaluation of facial nerve regeneration in rats using a motor nerve conduction test (electromyography). METHODS: Twenty-two rats were submitted to neurophysiological evaluation using motor nerve conduction of the mandibular branch of the facial nerve to obtain the compound muscle action potentials (CMAPs). To record the CMAPs, we used two needle electrodes that were inserted into the lower lip muscle of the rat. A supramaximal electrical stimulus was applied, and the values of CMAP latency, amplitude, length, area, and stimulus intensity obtained from each side were compared by use of the Wilcoxon test. RESULTS: There was no significant difference (all p > 0.05) in latency, amplitude, duration, area, or intensity of stimuli between the two sides. The amplitudes ranged between 1.61 and 8.30 mV, the latencies between 1.03 and 1.97 ms, and the stimulus intensities between 1.50 and 2.90 mA. CONCLUSIONS: This is a noninvasive, easy, and highly reproducible method that contributes to an improvement of the techniques previously described and may contribute to future studies of the degeneration and regeneration of the facial nerve.
OBJECTIVES: Our objective was to develop an experimental model for the noninvasive and objective evaluation of facial nerve regeneration in rats using a motor nerve conduction test (electromyography). METHODS: Twenty-two rats were submitted to neurophysiological evaluation using motor nerve conduction of the mandibular branch of the facial nerve to obtain the compound muscle action potentials (CMAPs). To record the CMAPs, we used two needle electrodes that were inserted into the lower lip muscle of the rat. A supramaximal electrical stimulus was applied, and the values of CMAP latency, amplitude, length, area, and stimulus intensity obtained from each side were compared by use of the Wilcoxon test. RESULTS: There was no significant difference (all p > 0.05) in latency, amplitude, duration, area, or intensity of stimuli between the two sides. The amplitudes ranged between 1.61 and 8.30 mV, the latencies between 1.03 and 1.97 ms, and the stimulus intensities between 1.50 and 2.90 mA. CONCLUSIONS: This is a noninvasive, easy, and highly reproducible method that contributes to an improvement of the techniques previously described and may contribute to future studies of the degeneration and regeneration of the facial nerve.
Authors: Oğuz Kadir Eğilmez; Numan Kökten; Mustafa Baran; M Tayyar Kalcıoğlu; Işın Doğan Ekici; Muhammet Tekin Journal: J Int Adv Otol Date: 2018-08 Impact factor: 1.017
Authors: Ricardo Ferreira Bento; Raquel Salomone; Silvia Bona do Nascimento; Ricardo Jose Rodriguez Ferreira; Ciro Ferreira da Silva; Heloisa Juliana Zabeu Rossi Costa Journal: Int Arch Otorhinolaryngol Date: 2014-05-23
Authors: Damon S Cooney; Eric G Wimmers; Zuhaib Ibrahim; Johanna Grahammer; Joani M Christensen; Gabriel A Brat; Lehao W Wu; Karim A Sarhane; Joseph Lopez; Christoph Wallner; Georg J Furtmüller; Nance Yuan; John Pang; Kakali Sarkar; W P Andrew Lee; Gerald Brandacher Journal: Sci Rep Date: 2016-08-11 Impact factor: 4.379
Authors: Numan Kökten; Oğuz Kadir Eğilmez; M Tayyar Kalcıoğlu; Mustafa Baran; A Işın Doğan Ekici Journal: Clin Exp Otorhinolaryngol Date: 2018-03-29 Impact factor: 3.372