| Literature DB >> 22528442 |
Matt D Medlin1, Joan M Taylor, Christopher P Mack.
Abstract
The GPCR-coupled sphingosine-1-phosphate (S1P) receptors regulate a number of important cell -functions, including proliferation, migration, and adhesion. Since these processes require dynamic regulation of the actin cytoskeleton, the ability to monitor S1P-dependent activation of the Rho family GTPases is critical for our understanding of S1P signaling. Herein, we provide methods for the GST pull-down-based assay used to measure Rho, Rac, and Cdc42 activity in cultured cells treated with S1P.Entities:
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Year: 2012 PMID： 22528442 PMCID： PMC6219392 DOI： 10.1007/978-1-61779-800-9_8
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745