| Literature DB >> 22528435 |
Leyre Brizuela1, Olivier Cuvillier.
Abstract
Sphingolipids (ceramide, sphingosine, and sphingosine-1-phosphate) are bioactive lipids with important biological functions in proliferation, apoptosis, angiogenesis, and inflammation. Herein, we describe easy and rapid biochemical methods with the use of radiolabeled molecules ((3)H, (32)P) for their mass determination. Quantitation of sphingosine kinase-1 activity, the most studied isoform, is also included.Entities:
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Year: 2012 PMID： 22528435 DOI： 10.1007/978-1-61779-800-9_1
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745